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       Ñîäåðæàíèå áèáëèîòåêè:

 

Óòâåðæäàþ

Çàìåñòèòåëü Ãëàâíîãî

ãîñóäàðñòâåííîãî

ñàíèòàðíîãî âðà÷à ÑÑÑÐ

Â.Å.ÊÎÂØÈËÎ

25 àâãóñòà 1983 ã. N 2874-83

 

ÌÅÒÎÄÈ×ÅÑÊÈÅ ÓÊÀÇÀÍÈß

ÏÎ ÏÐÈÌÅÍÅÍÈÞ ÍÎÐÌ ÐÀÑÕÎÄÀ ÄÈÀÃÍÎÑÒÈ×ÅÑÊÈÕ ÏÈÒÀÒÅËÜÍÛÕ

ÑÐÅÄ ÏÐÈ ÏÐÎÂÅÄÅÍÈÈ ÁÀÊÒÅÐÈÎËÎÃÈ×ÅÑÊÈÕ ÈÑÑËÅÄÎÂÀÍÈÉ

 

Ìåòîäè÷åñêèå óêàçàíèÿ ðàçðàáîòàíû Ãëàâíûì ñàíèòàðíî-ýïèäåìèîëîãè÷åñêèì óïðàâëåíèåì Ìèíèñòåðñòâà çäðàâîîõðàíåíèÿ ÑÑÑÐ (Þ.È. Ñàâåëüåâà), Îòäåëîì ïåðñïåêòèâíîãî ïëàíèðîâàíèÿ ñàíýïèäñëóæáû Èíñòèòóòà ìåäèöèíñêîé ïàðàçèòîëîãèè è òðîïè÷åñêîé ìåäèöèíû èì. Å.È. Ìàðöèíîâñêîãî (Ç.Ã. Íèêîëüñêàÿ, Í.Â. Êîæîêà).

 Ìåòîäè÷åñêèõ óêàçàíèÿõ ó÷òåíû ïðåäëîæåíèÿ Ìèíçäðàâîâ ñîþçíûõ ðåñïóáëèê, à òàêæå Ëåíèíãðàäñêîé ãîðîäñêîé ñàíýïèäñòàíöèè.

Ìåòîäè÷åñêèå óêàçàíèÿ ïðåäíàçíà÷åíû äëÿ áàêòåðèîëîãè÷åñêèõ ëàáîðàòîðèé ñàíèòàðíî-ýïèäåìèîëîãè÷åñêèõ ñòàíöèé.

 

1. ÎÁÙÈÅ ÏÎËÎÆÅÍÈß

 

Ìåòîäè÷åñêèå óêàçàíèÿ ïðåäíàçíà÷åíû äëÿ îïðåäåëåíèÿ ïîòðåáíîñòè â äèàãíîñòè÷åñêèõ ïèòàòåëüíûõ ñðåäàõ ïðè ïðîâåäåíèè áàêòåðèîëîãè÷åñêèõ è ñàíèòàðíî-áàêòåðèîëîãè÷åñêèõ èññëåäîâàíèé.

 

2. ÌÅÒÎÄÈÊÀ ÐÀÑ×ÅÒÀ ÏÎÒÐÅÁÍÎÑÒÈ Â ÏÐÅÏÀÐÀÒÀÕ

 

2.1. Íàñòîÿùàÿ Ìåòîäèêà ñîñòàâëåíà ñ ó÷åòîì äåéñòâóþùåé íîðìàòèâíî-òåõíè÷åñêîé äîêóìåíòàöèè.

2.2. Ïîäñ÷åò ïîòðåáíîñòè â ñðåäàõ ïðîèçâîäèòñÿ íà îïðåäåëåííîå ÷èñëî èññëåäîâàíèé (1000 è 100) ñ ó÷åòîì âûïîëíåííîãî êîëè÷åñòâà èëè ïðåäïîëàãàåìîãî ïî ôîðìóëå:

 

                                À x ã

                         x = ----------,

                             1000 (100)

 

ãäå:

x - êîëè÷åñòâî ñðåäû â ã, êã;

À - ÷èñëî âûïîëíåííûõ àíàëèçîâ â ãîä;

ã - íîðìà ðàñõîäà ñðåäû íà 1000 (100) àíàëèçîâ.

2.3. Ðàñ÷åòíûå ïîòðåáíîñòè ãðóïïèðóþòñÿ ïî âèäàì èíôåêöèé (ýïèäåìèîëîãè÷åñêèå áàêèññëåäîâàíèÿ) è îáúåêòàì (ñàíèòàðíî-áàêòåðèîëîãè÷åñêèå èññëåäîâàíèÿ).

2.4. Ìåòîäè÷åñêèå óêàçàíèÿ ïðåäóñìàòðèâàþò ïåðå÷åíü ñðåä ïðîèçâîäñòâåííîãî è ëàáîðàòîðíîãî èçãîòîâëåíèÿ.

2.5. Ïðè ñîñòàâëåíèè çàÿâêè íà äèàãíîñòè÷åñêèå ñðåäû íåîáõîäèìî ðóêîâîäñòâîâàòüñÿ äàííûìè Ìåòîäè÷åñêèìè óêàçàíèÿìè.

2.6. Ïðè îáîçíà÷åíèè êîëè÷åñòâ ñðåä, ðåàêòèâîâ â ñêîáêàõ ïðèâåäåíû íàèìåíîâàíèÿ åäèíèö ôèçè÷åñêèõ âåëè÷èí â ñîîòâåòñòâèè ñ ÃÎÑÒ 8.417-81 (ÑÒ ÑÝ 1052-78) "ÃÑÈ. Åäèíèöû ôèçè÷åñêèõ âåëè÷èí".

 

I. Ïðèìåðíûé ðàñ÷åò ðàñõîäà ïèòàòåëüíûõ ñðåä

ïðè ïðîâåäåíèè áàêòåðèîëîãè÷åñêèõ èññëåäîâàíèé

 

┌────┬────────────┬─────────────────────────────────┬──────────────────────────┐

│ N  │Íàèìåíîâàíèå│       Íàèìåíîâàíèå ñðåäû             Êîëè÷åñòâî ñðåäû,   

ï/ï │  ðàçäåëà                                    │ìë (êóá. ñì), ë (êóá. äì),│

       ðàáîòû                                    ã, êã íà 1000 èññëåäîâàíèé│

├────┼────────────┼─────────────────────────────────┼──────────────────────────┤

│ 1       2                      3                            4            

├────┼────────────┼─────────────────────────────────┼──────────────────────────┤

│1.  │Êèøå÷íûå    │Áàêòîàãàð Ïëîñêèðåâà ñóõîé       │1,4 êã <*>               

    │èíôåêöèè    │Àãàð Ýíäî ñóõîé                  │1,2 êã                    

                │Àãàð ñ ýîçèí-ìåòèëåíîâûì ñèíèì   │1,2 êã                   

                │ñóõîé (ñðåäà Ëåâèíà)                                      

                │Âèñìóò-ñóëüôèò àãàð ñóõîé        │1,4 êã                   

                │Ïèòàòåëüíûé àãàð                 │3,0 ë (êóá. äì)          

                │Ñðåäà ñ èíäèêàòîðîì ÂÐ è îäíèì èç│3,0 ë (êóá. äì)          

                │óãëåâîäîâ (ãëþêîçà, ëàêòîçà,                              

                │ìàëüòîçà, ñàõàðîçà) èëè                                   

                │ìíîãîàòîìíûì ñïèðòîì ìàííèòîì                             

                │Ïèòàòåëüíàÿ ñðåäà Ðåññåëÿ ñóõàÿ  │0,25 êã                  

                │Ïèòàòåëüíàÿ ñðåäà äëÿ ïåðâè÷íîé  │0,3 êã                   

                │äèôôåðåíöèàöèè ýíòåðîáàêòåðèé                             

                │ñóõàÿ (òèïà Êëèãëåðà)                                     

                │Ïèòàòåëüíàÿ ñðåäà ñóõàÿ,         │0,09 êã                  

                ïðåäíàçíà÷åííàÿ äëÿ îïðåäåëåíèÿ                           

                │B-ãàëàêòîçèäàçû ýíòåðîáàêòåðèé                            

                │Àãàð ùåëî÷íîé ñóõîé              │1,2 êã <**>              

                │Àãàð ùåëî÷íîé äðîææåâîé ñóõîé    │3,5 êã <**>              

                │Ïåïòîí îñíîâíîé ñóõîé            │0,42 êã <**>             

                │Ïèòàòåëüíàÿ ñðåäà äëÿ îïðåäåëåíèÿ│1,2 êã (íà 1000 âûäåëåííûõ│

                │÷óâñòâèòåëüíîñòè ìèêðîîðãàíèçìîâ │êóëüòóð)                 

                │ê àíòèáèîòèêàì ñóõàÿ (ÀÃÂ)                                

                │Õëîðèñòî-ìàãíèåâàÿ ñðåäà         │5,0 ë (êóá. äì)           

                │Æåë÷íûé áóëüîí 10%               │5,0 ë (êóá. äì)          

                │Ñóõîé ôåðìåíòàòèâíûé ãèäðîëèçàò  │20 ã íà 1 ë (êóá. äì)    

                │êàçåèíà íåãëóáîêîé ñòåïåíè       │ñðåäû                    

                │ðàñùåïëåíèÿ                                               

                │Êèñëîòíûé ãèäðîëèçàò êàçåèíà     │15 - 20 ã íà 1 ë (êóá. äì)│

                │ñðåäíåé ñòåïåíè ðàñùåïëåíèÿ ñóõîé│ñðåäû                    

                │Ìÿñîïåïòîííûé áóëüîí             │10,0 ë (êóá. äì)         

                │Ìÿñîïåïòîííûé àãàð               │6,0 ë (êóá. äì)          

                │Òðåõñàõàðíûé àãàð ñ ìî÷åâèíîé    │7,0 ë (êóá. äì)          

                │(ñðåäà Îëüêåíèöêîãî)                                      

                │Ñðåäû óãëåâîäíûå æèäêèå ñ        │4,0 ë (êóá. äì) êàæäîé   

                │ïîïëàâêàìè (êñèëîçà, àðàáèíîçà,                           

                │äóëüöèò è ò.ä.)                                           

                │Ãëèöåðèíîâûé àãàð                │4,0 ë (êóá. äì)          

                │Ïåïòîííàÿ âîäà 1% äëÿ îïðåäåëåíèÿ│5,0 ë (êóá. äì)          

                │èíäîëà                                                     

                │Ïîëóæèäêèé àãàð 0,3% äëÿ         │4,0 ë (êóá. äì)          

                │îïðåäåëåíèÿ ïîäâèæíîñòè                                   

                │Ñðåäà Êðèñòåíñåíà                │3,0 ë (êóá. äì)           

                │Ñðåäà îáîãàùåíèÿ ñ ñåëåíèòîì     │5,0 ë (êóá. äì)          

                │íàòðèÿ èëè                                                

                │Ñðåäà îáîãàùåíèÿ Ìþëëåðà         │10,0 ë (êóá. äì)         

                │Ñðåäà Ñèìîíñà                    │4,0 ë (êóá. äì)          

                │Ãëèöåðèíîâàÿ ñìåñü               │5,0 ë (êóá. äì)          

                │(êîíñåðâèðóþùàÿ æèäêîñòü Òèãå â                           

                │ìîäèôèêàöèè Çàêñà)                                        

                │Áóëüîí ñ ìî÷åâèíîé               │1,0 ë (êóá. äì)          

                │Áóëüîí Õîòòèíãåðà                │5,0 ë (êóá. äì)          

                │Ôåíèëàëàíèíîâûé àãàð             │2,0 ë (êóá. äì)          

                │Ñðåäà ñ ìàëîíàòîì íàòðèÿ         │2,0 ë (êóá. äì)          

                │Ñðåäà ñ ëèçèíîì (äëÿ îïðåäåëåíèÿ │2,0 ë (êóá. äì)          

                │ëèçèíäåêàðáîêñèëàçû)                                      

                │Ñðåäà ñ äóëüöèòîì                │2,0 ë (êóá. äì)          

                │Ñðåäà Êëàðêà                     │3,0 ë (êóá. äì)          

                │Ñðåäà Ñåðîâà (æèäêàÿ)            │5,0 ë (êóá. äì)          

                │Ñðåäà Ñåðîâà (ïëîòíàÿ)           │2,5 êã                   

                │Ñðåäà ñ ìî÷åâèíîé (ïî Ïðåóñó)    │3,0 ë (êóá. äì)          

                │Ïèòàòåëüíûé áóëüîí ñóõîé         │0,2 êã                   

│2.  │Äèôòåðèÿ    │Ñûâîðîòêà êðóïíîãî ðîãàòîãî ñêîòà│2,0 ë (êóá. äì)          

                │áåç êîíñåðâàíòà èëè íîðìàëüíàÿ                            

                │ëîøàäèíàÿ ñûâîðîòêà                                        

                │Ñûâîðîòêà êðóïíîãî ðîãàòîãî ñêîòà│3,0 ë (êóá. äì)          

                │ñ êîíñåðâàíòîì                                            

                │Ñðåäà Êëàóáåðãà II               │6,0 ë (êóá. äì)           

                │Êðîâü äëÿ ñðåäû Êëàóáåðãà II     │3,5 ë (êóá. äì)          

                │Ñóõîé ïèòàòåëüíûé àãàð äëÿ ñðåäû │0,5 êã                   

                │Êëàóáåðãà II                                              

                │Êðîâÿíîé òåëëóðèòîâûé àãàð       │6,0 ë (êóá. äì)          

                │2% ðàñòâîð òåëëóðèòà êàëèÿ       │400 ìë (êóá. ñì)         

                                                 │80 ôëàêîíîâ              

                │Êðîâü äëÿ 5% êðîâÿíîãî àãàðà     │1,7 ë (êóá. äì)          

                Ýëåêòèâíàÿ õèíîçîëüíàÿ           │1,0 êã                   

                │èíäèêàòîðíàÿ ïèòàòåëüíàÿ ñðåäà                            

                │äëÿ ìèêðîáèîëîãè÷åñêîé                                    

                │äèàãíîñòèêè äèôòåðèè (ñðåäà                               

                │Áó÷èíà) ñóõàÿ                                             

                │Öèñòèí-òåëëóðèò ñûâîðîòî÷íàÿ     │6,0 ë (êóá. äì)          

                │ñðåäà Òèíåäàëÿ - Ñàäûêîâîé                                

                │Ñóõîé ïèòàòåëüíûé àãàð äëÿ ñðåäû │3,0 ë (êóá. äì)          

                │Ïèçó è 5% êðîâÿíîãî àãàðà                                  

                │Ñðåäû óãëåâîäíûå ñ ãëþêîçîé,     │ïî 2,0 ë (êóá. äì) êàæäîé │

                │ñàõàðîçîé, êðàõìàëîì             │ñðåäû                    

                │Ìàðòåíîâñêèé àãàð íà ìÿñíîé âîäå │1,0 ë (êóá. äì) (íà 1000 

                │äâîéíîé êîíöåíòðàöèè äëÿ         │âûäåëåííûõ êóëüòóð)      

                │îïðåäåëåíèÿ òîêñèãåííîñòè                                 

                │Ìàðòåí-öèñòèí-àãàðîâàÿ ñðåäà ñ   │0,2 ë (êóá. äì)          

                │äîáàâëåíèåì íîðìàëüíîé ñûâîðîòêè │                         

                │(ëîøàäèíîé èëè êðóïíîãî ðîãàòîãî │                         

                │ñêîòà)                                                    

                │Ìàðòåí-öèñòèí-àãàðîâàÿ ñðåäà áåç │0,2 ë (êóá. äì)          

                │äîáàâëåíèÿ íàòèâíîé ñûâîðîòêè                             

                │Ñðåäà îáîãàùåíèÿ                 │1,0 ë (êóá. äì)          

                │Ïèòàòåëüíûé àãàð äëÿ îïðåäåëåíèÿ │1,0 êã (íà 1000 âûäåëåííûõ│

                │òîêñèãåííîñòè äèôòåðèéíûõ        │êóëüòóð)                 

                │ìèêðîîðãàíèçìîâ ñóõîé (ÎÒÄÌ)                              

                │Áóëüîí ñ ìî÷åâèíîé               │1,0 ë (êóá. äì)          

                │Ìàðòåíîâñêèé áóëüîí              │4,0 ë (êóá. äì) (íà 1000 

                                                 │âûäåëåííûõ êóëüòóð)      

│3.  │Êîêëþø,     │Êàçåèíîâî-óãîëüíûé àãàð          │2,0 êã                   

    │ïàðàêîêëþø  │Ñðåäà ñ òèðîçèíîì                │2,0 ë (êóá. äì)          

                │ÌÊÀ (àãàð-àãàð äàëüíåâîñòî÷íûé)  │3,5 êã                   

                │Ñðåäà Ñèìîíñà                    │4,0 ë (êóá. äì)          

│4.  │Ìåíèíãîêîê- │Òèàìèí-öèñòèí ãëþòàìèíîâûé àãàð  │2,0 ë (êóá. äì)          

    │êîâàÿ       │ñóõîé                                                     

    │èíôåêöèÿ    │Ïèòàòåëüíûé àãàð                 │2,0 ë (êóá. äì)          

                │Ñðåäû ñ óãëåâîäàìè (ãëþêîçà,     │2,0 ë (êóá. äì) (íà 1000 

                │ìàëüòîçà, ñàõàðîçà, ëàêòîçà,     │âûäåëåííûõ êóëüòóð)      

                │ôðóêòîçà)                                                  

                │5% æåë÷íûé àãàð                  │2,0 ë (êóá. äì) (íà 1000 

                                                 │âûäåëåííûõ êóëüòóð)      

                │Ñûâîðîòî÷íàÿ ñðåäà ñ ëèíêîìèöèíîì│2,0 ë (êóá. äì)          

                │Ñûâîðîòî÷íûé àãàð                │3,0 ë (êóá. äì)          

                │Ñðåäà Õüþ Ëåéôñîíà               │10,0 ë (êóá. äì)         

                │Ñûâîðîòêà êðóïíîãî ðîãàòîãî ñêîòà│0,5 ë (êóá. äì)          

                │áåç êîíñåðâàíòà                                           

                │Ïîëóæèäêèé 0,1% àãàð             │5,0 ë (êóá. äì) (íà 1000 

                                                 │âûäåëåííûõ êóëüòóð)      

                │Ïèòàòåëüíûé áóëüîí ñóõîé         │0,2 êã                   

│5.  │Ñòàôèëîêîê- │Ìÿñîïåïòîííûé àãàð (ÌÏÀ)         │2,0 êã                   

    │êîâàÿ       │Ñðåäà ñ èíäèêàòîðîì ÂÐ è         │2,0 ë (êóá. äì)          

    │èíôåêöèÿ    │ìíîãîàòîìíûì ñïèðòîì ìàííèòîì                             

                │Êðîëè÷üÿ ïëàçìà                  │0,2 ë (êóá. äì) (íà 1000 

                                                 │âûäåëåííûõ êóëüòóð)      

                │Ñðåäà ×èñòîâè÷à                  │4,0 ë (êóá. äì)          

                │Ñîëåâîé áóëüîí 10%               │4,0 ë (êóá. äì)          

                │Ñîëåâîé áóëüîí 5%                │4,0 ë (êóá. äì)          

                │Ìîëî÷íî-ñîëåâîé àãàð             │2,0 ë (êóá. äì)           

                │Êðîâÿíîé àãàð                    │3,0 ë (êóá. äì)          

                │Ñàõàðíûé áóëüîí                  │4,0 ë (êóá. äì)          

                │Ïëàçìà (êðîëè÷üÿ, ÷åëîâå÷åñêàÿ)  │0,2 ë (êóá. äì) (íà 1000 

                                                 │âûäåëåííûõ êóëüòóð)      

                │Àãàð ýëåêòèâíûé ñîëåâîé äëÿ      │5,0 ë (êóá. äì)          

                │ñòàôèëîêîêêîâ                                             

│6.  │Ãíîéíî-     │Êðîâÿíîé àãàð                    │3,0 ë (êóá. äì)          

    │ñåïòè÷åñêèå │Ñðåäà äëÿ êîíòðîëÿ ñòåðèëüíîñòè  │20,0 ë (êóá. äì)         

    │çàáîëåâàíèÿ │(òèîãëèêîëåâàÿ ñðåäà)                                     

                │Ñðåäà Ñàáóðî (æèäêàÿ)            │10,0 ë (êóá. äì)         

                │Ñàõàðíûé áóëüîí                  │10,0 ë (êóá. äì)         

                │Ñðåäà "6-Á"                      │20,0 ë (êóá. äì)         

                │Ñðåäà À-2 (ïëîòíàÿ)              │12,0 ë (êóá. äì)         

                │Ñðåäà À-1 (æèäêàÿ)               │3,0 ë (êóá. äì)          

                │Ñðåäà Ê-1 (íà êëåáñèåëÿ)         │3,0 ë (êóá. äì)          

                │Ñðåäà Ê-2 (íà êëåáñèåëÿ)         │12,0 ë (êóá. äì)         

                │Øîêîëàäíûé àãàð Ëåâèíòàëÿ        │20,0 ë (êóá. äì)         

                │Ñðåäà ÖÏÕ (íà ñèíåãíîéíóþ        │12,0 ë (êóá. äì)         

                │ïàëî÷êó)                                                  

└────┴────────────┴─────────────────────────────────┴──────────────────────────┘

 

--------------------------------

<*> Ïðè ïåðåñåâå ñî ñðåä îáîãàùåíèÿ êîëè÷åñòâî ñðåäû ìîæåò áûòü óâåëè÷åíî äî 2,8 êã.

<**> Ïðè âûïîëíåíèè èññëåäîâàíèé íà ô. 30 êîëè÷åñòâî ñðåä ìîæåò áûòü óâåëè÷åíî äî 2,8 êã.

 

II. Áàêòåðèîëîãè÷åñêèé àíàëèç êðîâè íà ãåìîêóëüòóðó

 

┌────┬──────────────────────────────────────────────┬──────────────────────────┐

│ N                Íàèìåíîâàíèå ñðåäû                   Êîëè÷åñòâî ñðåäû    

ï/ï │                                                 íà 100 èññëåäîâàíèé   

                                                        (ìë, ã, êã, ë)     

├────┼──────────────────────────────────────────────┼──────────────────────────┤

│1.  │Ñðåäà Ðàïîïîðò èëè                            │5,0 ë (êóá. äì)          

    │Æåë÷íûé áóëüîí 20%                            │5,0 ë (êóá. äì)          

│2.  │Àãàð Ýíäî ñóõîé èëè àãàð ñ ýîçèí-ìåòèëåíîâûì  │2,0 ë (êóá. äì)          

    │ñèíèì (ñðåäà Ëåâèíà)                                                   

│3.  │Ñðåäà Ðåññåëÿ                                 │0,5 ë (êóá. äì)          

│4.  Ñðåäû óãëåâîäíûå æèäêèå ñ ïîïëàâêîì (ãëþêîçà, │ïî 0,5 ë (êóá. äì) êàæäîé │

    │ëàêòîçà, ìàííèò, ñàõàðîçà)                    │ñðåäû                    

│5.  │Ïèòàòåëüíûé àãàð                              │4,0 ë (êóá. äì)          

│6.  │Ïåïòîííàÿ âîäà 1%                             │0,1 ë (êóá. äì)          

│7.  │Ïîëóæèäêèé àãàð                               │0,3 ë (êóá. äì)          

│8.  │Ñðåäà Êðèñòåíñåíà                             │3,0 ë (êóá. äì)          

│9.  │Âèñìóò-ñóëüôèò àãàð ñóõîé                     │2,0 ë (êóá. äì)          

│10. │Áóëüîí Øòåðíà                                 │0,5 ë (êóá. äì)          

│11. │Ñðåäà Ñèìîíñà                                 │0,4 ë (êóá. äì)          

│12. │Àöåòàòíûé àãàð                                │0,4 ë (êóá. äì)          

│13. │Ñðåäà ñ ëèçèíîì                                                        

│14. │Ñðåäà ñ äóëüöèòîì                             │0,2 ë (êóá. äì)          

└────┴──────────────────────────────────────────────┴──────────────────────────┘

 

III. Ïðèìåðíûé ðàñ÷åò ïèòàòåëüíûõ ñðåä ïðè ïðîèçâîäñòâå

ñàíèòàðíî-áàêòåðèîëîãè÷åñêèõ èññëåäîâàíèé (íà 100 àíàëèçîâ)

 

┌────┬──────────────────┬──────────────┬───────────────┬──────────────┬────────┐

│ N     Íàèìåíîâàíèå   Íàèìåíîâàíèå │ Îáùåå êîë-âî  │ Íàèìåíîâàíèå │ Êîë-âî │

│ï/ï │  ðàçäåëà ðàáîòû      ñðåäû          ñðåäû     │ èíãðåäèåíòîâ │       

├────┼──────────────────┼──────────────┼───────────────┼──────────────┼────────┤

│ 1          2               3              4             5          6   

├────┼──────────────────┼──────────────┼───────────────┼──────────────┼────────┤

│1.  │Áàêòåðèîëîãè÷åñêèé│Ãëþêîçî-      │5 ë (êóá. äì)  │ïåïòîí        │0,5 êã 

    │àíàëèç âîäû íà    │ïåïòîííàÿ                    │NaCl          │0,25 êã │

    │êîëè-òèòð è       │ñðåäà (êîëè-                 │ãëþêîçà       │0,25 êã │

    áàêòåðèàëüíóþ     │òèòð 500)                                         

    │îáñåìåíåííîñòü,   │Ãëþêîçî-      │3,5 ë (êóá. äì)│ïåïòîí        │0,35 êã │

    │âêëþ÷àÿ è         │ïåïòîííàÿ                    │NaCl          │0,175 êã│

    │ìèíåðàëüíûå       │ñðåäà, êîëè-                 │ãëþêîçà       │0,175 êã│

    │(íàòóðàëüíûå âîäû,│òèòð 300                                          

    │áåçàëêîãîëüíûå    │Ãëþêîçî-      │1,2 ë (êóá. äì)│ïåïòîí        │0,12 êã │

    │íàïèòêè è ñèðîïû) │ïåïòîííàÿ                    │NaCl          │0,06 êã │

                      │ñðåäà, êîëè-                 │ãëþêîçà       │0,06 êã │

                      │òèòð III                                           

                      │(êîëîäåçíàÿ                                       

                      │âîäà)                                             

    │à) áðîäèëüíûì     │Ïèòàòåëüíûé   │3,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,105 êã│

    │(òèòðàöèîííûì)    │àãàð                                              

    │ìåòîäîì è ìåòîäîì │Ñðåäà Ýíäî    │4,5 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,180 êã│

    │ÐÄÀ èç èñòî÷íèêîâ │Ñðåäà ñ       │ïî 0,2 ë (êóá. │ïåïòîí        │2,0 ã  

    │öåíòðàëèçîâàííîãî │èíäèêàòîðîì ÂÐ│äì) êàæäîé     │NaCl          │1,0 ã  

    │âîäîñíàáæåíèÿ     │è óãëåâîäàìè                 │óãëåâîäà             

    │(êîëè-òèòð 500)   │(ãëþêîçîé èëè │               │(êàæäîãî)            

                      │ìàííèòîì,                                         

                      │ëàêòîçîé)                                         

                      │Ñèñòåìû                                    │2 íàáîðà│

                      │èíäèêàòîðíûå                                      

                      │áóìàæíûå äëÿ                                      

                      │ïîñòàíîâêè                                        

                      │îêñèäàçíîãî                                       

                      │òåñòà è                                           

                      │ôåðìåíòàöèè                                       

                      │ãëþêîçû                                           

    │á) ìåòîäîì        Ìåìáðàííûå    │500 øò.        │ìåìáðàííûå    │500 øò. │

    │ìåìáðàííûõ        │ôèëüòðû                      ôèëüòðû N 3          

    │ôèëüòðîâ          │Ñðåäà Ýíäî    │2,5 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,1 êã 

                      │Ñðåäà ñ       │ïî 0,2 ë (êóá. │ïåïòîí        │2,0 ã  

                      │èíäèêàòîðîì ÂÐ│äì) êàæäîé     │NaCl          │1,0 ã  

                      │è óãëåâîäàìè  │ñðåäû          │óãëåâîäà      │2,0 ã  

                      │(ãëþêîçîé èëè │               │(êàæäîãî)            

                      │ìàííèòîì,                                         

                      │ëàêòîçîé)                                         

│2.  Áàêàíàëèç âîäû (íà│Ãëþêîçî-      │1,2 ë (êóá. äì)│ïåïòîí        │0,12 êã │

    êîëè-òèòð è       │ïåïòîííàÿ                    │NaCl          │0,06 êã │

    │áàêîáñåìåíåííîñòü)│ñðåäà (ÃÏÑ)                  │ãëþêîçà       │0,06 êã │

    │ðåê, îçåð, ïðóäîâ,│Ïèòàòåëüíûé   │3,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,105 êã│

    │ìîðåé, ñòî÷íîé    │àãàð                                              

    │âîäû, òåõíè÷åñêîé │Ñðåäà ñ       │ïî 0,2 ë (êóá. │ïåïòîí        │2,0 ã  

    │âîäû              │èíäèêàòîðîì ÂÐ│äì) êàæäîé     │NaCl          │1,0 ã  

                      │è îäíèì èç    │ñðåäû          │óãëåâîäà      │2,0 ã  

                      │óãëåâîäîâ                    │(êàæäîãî)            

                      │(ãëþêîçà èëè                                      

                      │ìàííèò,                                           

                      │ëàêòîçà)                                          

                      │Ñðåäà Ýíäî    │4,5 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,180 êã│

                      │Ñðåäà Êîäà    │0,5 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,016 êã│

                      │Ñðåäà         │0,4 ë (êóá. äì)│àãàð-àãàð     │7,5 ã  

                      │×èñòîâè÷à                    │ïåïòîí        │4,5 ã  

                                                   │NaCl          │37,5 ã 

                                                   │æåëòîê êóð.   │1 øò.  

                                                   │ÿéöà                 

│3.  │Èññëåäîâàíèå âîäû │Õëîðèñòî-     │5,0 ë (êóá. äì)│ìàãíèé        │195,0 ã │

    │íà ïàòîãåííóþ     │ìàãíèåâàÿ                    │õëîðèñòûé            

    │ôëîðó (áðþøíîé    │ñðåäà                        │êðèñòàëë.            

    │òèô, äèçåíòåðèÿ,                               │íàòðèé        │40,0 ã 

    │ÝÏÊÏ)                                          │õëîðèñòûé            

                                                   │êàëèé         │8,0 ã  

                                                   │ôîñôîðíîêèñëûé│       

                                                   │îäíîçàìåùåííûé│       

                                                   │ïåïòîí 10%    │250,0 ìë│

                                                   │ðàñòâîð       │(êóá.  

                                                                 ñì)    

                                                   │äðîææåâîé     │110,0 ìë│

                                                   │ýêñòðàêò      │(êóá.  

                                                                 ñì)    

                                                   │áðèëëèàíòîâûé │25,0 ìë │

                                                   │çåëåíûé 0,1%  │(êóá.  

                                                   │âîäíûé ðàñòâîð│ñì)    

                      Ñåëåíèòîâàÿ   │5,0 ë (êóá. äì)│íàòðèé êèñëûé │20,0 ã 

                      │ñðåäà                        │ñåëåíèñòîêèñ- │       

                                                   │ëûé NaHSeO           

                                                             3          

                                                   │ïåïòîí        │25,0 ã 

                                                   │íàòðèé        │15,0 ã 

                                                   │ôîñôîðíîêèñëûé│       

                                                   │äâóçàìåùåííûé │       

                                                   │áåçâîäíûé            

                                                   │NaH PO               

                                                      2  4              

                                                   │ëàêòîçà       │20,0 ã 

                                                   │íàòðèé        │25,0 ã 

                                                   │ôîñôîðíîêèñëûé│       

                                                   │îäíîçàìåùåííûé│       

                                                   │Na HPO               

                                                     2   4              

                      │Ñðåäà Ýíäî èëè│14,0 ë (êóá.   │ñóõàÿ ñðåäà   │0,56 êã │

                      │Ëåâèíà        │äì)                                 

                      │Âèñìóò-ñóëüôèò│14,0 ë (êóá.   │ñóõàÿ ñðåäà   │0,91 êã │

                      │àãàð          │äì)                                 

                      │Òðåõñàõàðíûé  │5,0 ë (êóá. äì)│ñîëü Ìîðà     │1,0 ã  

                      │àãàð (ñðåäà                  │ãèïîñóëüôèò   │1,5 ã  

                      │Îëüêåíèöêîãî) │               │íàòðèÿ               

                                                   │ïåïòîí        │0,1 êã 

                      │Ñðåäà         │14,0 ë (êóá.   │ñóõàÿ ñðåäà   │0,84 êã │

                      │Ïëîñêèðåâà    │äì)                                 

                      │Ñðåäà ñ       │4,0 ë (êóá. äì)│îõìåëåííîå    │40,0 ã 

                      îõìåëåííûì                   │ñóñëî                

                      │ñóñëîì                       │ïåïòîí        │200,0 ã │

                      │Æåë÷íûé áóëüîí│25,0 ë (êóá.   │æåë÷ü         │2,5 ã  

                      │10%           │äì)            │ìÿñîïåïòîííûé │22,5 ë 

                                                   │áóëüîí        │(êóá.  

                                                                 │äì)    

│4.  │Áàêòåðèîëîãè÷åñêèé│Ñðåäà Êåññëåðà│0,5 ë (êóá. äì)│ïåïòîí        │5,0 ã  

    │àíàëèç ñìûâîâ íà                               │æåë÷ü         │25,0 ã 

    │ñàíèòàðíî-                                     │ëàêòîçà       │1,25 ã 

    │ïîêàçàòåëüíûå                                  │ãåíöèàí-      │0,01 ã 

    │ìèêðîîðãàíèçìû è                               ôèîëåòîâûé           

    │áàêòåðèàëüíóþ     │Ñðåäà Ýíäî    │0,5 ë (êóá. äì)│ñóõàÿ ñðåäà   │20,0 ã 

    │îáñåìåíåííîñòü    │Ñðåäà Êîäà    │0,5 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,016 ã │

                      │Ñðåäà Õåéôåöà │0,5 ë (êóá. äì)│ïåïòîí        │5,0 ã  

                                                   │õëîðèäà íàòðèÿ│2,5 ã  

                                                   │ìàííèò        │2,5 ã  

                                                   │5% ñïèðòîâûé  │5,0 ìë 

                                                   │ð-ð ðîçîëîâîé │       

                                                   │êèñëîòû              

                                                   │0,1% âîäíûé ð-│11,5 ìë │

                                                   ð ìåòèëåíîâîãî│       

                                                   │ñèíåãî               

                      │Ñðåäà         │4,0 ë (êóá. äì)│ïåïòîí        │40,0 ã 

                      óãëåâîäíàÿ ñ                 │NaCl          │20,0 ã 

                      │ïîïëàâêàìè                   │óãëåâîäà      │40,0 ã 

                      │(ãëþêîçà)                                         

                      │Ñðåäû ñ       │ïî 0,2 ë (êóá. │ïåïòîí        │2,0 ã  

                      │èíäèêàòîðîì ÂÐ│äì) êàæäîé     │NaCl          │1,0 ã  

                      │è îäíèì èç    │ñðåäû          │óãëåâîäû      │2,0 ã  

                      │óãëåâîäîâ                    │(êàæäîãî)            

                      │(ãëþêîçîé èëè │                                    

                      │ìàííèòîì,                                         

                      │ëàêòîçîé)                                         

│5.  │Èññëåäîâàíèå      │Ñðåäà         │0,4 ë (êóá. äì)│àãàð-àãàð     │7,5 ã  

    │ñìûâîâ íà         │×èñòîâè÷à                    │ïåïòîí        │4,5 ã  

    │ñòàôèëîêîêê                                    │NaCl          │31,7 ã 

                                                   │æåëòîê        │1 øò.  

                                                   │êóðèíîãî ÿéöà │       

                                                   │ìÿñíàÿ âîäà   │0,2 ë  

                                                                 │(êóá.  

                                                                 │äì)    

                      │Ìîëî÷íî-      │2,0 ë (êóá. äì)│ñóõîé         │100,0 ã │

                      │ñîëåâîé àãàð                 │ïèòàòåëüíûé          

                                                   │àãàð                 

                                                   │NaCl          │130,0 ã │

                                                   ñíÿòîå        │0,2 ë  

                                                   │ñòåðèëüíîå    │(êóá.  

                                                   │ìîëîêî        │äì)    

                      │Ñîëåâîé áóëüîí│0,5 ë (êóá. äì)│ïåïòîí        │5,0 ã  

                      │10%                          │NaCl          │32,5 ã 

                      │Ñàõàðíûé      │0,5 ë (êóá. äì)│ïåïòîí        │5,0 ã  

                      │áóëüîí                       │NaCl          │3,0 ã  

                                                   │ãëþêîçà       │5,0 ã  

                      │Êðîâÿíîé àãàð │3,0 ë (êóá. äì)│ñóõîé         │150,0  

                                                   │ïèòàòåëüíûé          

                                                   │àãàð                 

                                                   │äåôèáðèíèðî-  │0,15 ë 

                                                   │âàííàÿ êðîâü  │(êóá.  

                                                                 │äì)    

                      │Ñîëåâîé àãàð  │3,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │327,6 ã │

                      │Êðîëè÷üÿ      │0,02 ë (êóá.   │(íà 100              

                      │ïëàçìà        │äì)            │âûäåëåííûõ           

                                                   │êóëüòóð)             

│6.  │Èññëåäîâàíèå      │Ïèòàòåëüíûé   │2,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,1 ã  

    │ñìûâîâ íà ïðîòåé  │àãàð                                              

                      │Àãàð ñ        │0,5 ë (êóá. äì)│àãàð-àãàð     │6,0 ã  

                      │ôåíèëàëàíèíîì │               │äðîææåâîé     │50 ìë  

                                                   │ýêñòðàêò      │(êóá.  

                                                                 ñì)    

                                                   │ôåíèëàëàíèí   │1,0 ã  

                                                   │Na HPO  x     │0,5 ã  

                                                     2   4              

                                                   │12H O                

                                                      2                 

                                                   │NaCl          │2,5 ã  

                      │Ñðåäà         │0,2 ë (êóá. äì)│NaCl          │0,5 ã  

                      │óãëåâîäíàÿ,                  │ìàëüòîçà      │1,0 ã  

                      │æèäêàÿ ñ                                          

                      │ìàëüòîçîé                                         

                      │Òðåõñàõàðíûé  │0,5 ë (êóá. äì)│ëàêòîçà       │5,0 ã  

                      │àãàð (ñðåäà                  │ñîëü Ìîðà     │0,1 ã  

                      │Îëüêåíèöêîãî) │               │ãèïîñóëüôèò   │0,15 ã 

                                                   │ñàõàðîçà      │5,0 ã  

                                                   │ãëþêîçà       │5,0 ã  

                                                   │ìî÷åâèíà      │5,0 ã  

                                                   │ð-ð ôåíîëîâîãî│2,0 ìë 

                                                   │êðàñíîãî      │(êóá.  

                                                                 ñì)    

│7.  │Áàêèññëåäîâàíèå   │Ñðåäà Ìþëëåðà │1,0 ë (êóá. äì)│ìåëà õ÷       │45,0 ã 

    │ñìûâîâ íà                                      │á-íà          │0,9 ë  

    │ïàòîãåííóþ ôëîðó                               │Õîòòèíãåðà    │(êóá.  

    │(ñàëüìîíåëëû,                                                │äì)    

    │äèçåíòåðèþ)                                    │ð-ðà Ëþãîëÿ   │0,02 ë 

                                                                 │(êóá.  

                                                                 │äì)    

                      Ñåëåíèòîâàÿ   │0,5 ë (êóá. äì)│íàòðèé êèñëûé │2,0 ã  

                      │ñðåäà                        │ñåëåíîñòîêèñ- │       

                                                   │ëûé                  

                                                   │ïåïòîí        │2,5 ã  

                                                   │íàòðèé        │1,5 ã  

                                                   │ôîñôîðíîêèñëûé│       

                                                   │äâóçàìåùåííûé │       

                                                   │áåçâîäíûé            

                                                   │ëàêòîçà õ÷    │2,0 ã  

                                                   │íàòðèé        │2,5 ã  

                                                   │ôîñôîðíîêèñëûé│       

                                                   │îäíîçàìåùåííûé│       

                      │Ñðåäà         │2,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,120 êã│

                      │Ïëîñêèðåâà                                        

                      │Ñðåäà âèñìóò- │2,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,120 êã│

                      │ñóëüôèòàãàð                                       

                      │Æåë÷íûé áóëüîí│1,0 ë (êóá. äì)│æåë÷ü         │0,2 ë  

                      │20%                                        │(êóá.  

                                                                 │äì)    

                                                   │ìÿñîïåïòîííûé │0,8 ë  

                                                   │áóëüîí        │(êóá.  

                                                                 │äì)    

                      │Õëîðèñòî-     │1,0 ë (êóá. äì)│ìàãíèé        │39,0 ã 

                      │ìàãíèåâàÿ                    │õëîðèñòûé            

                      │ñðåäà                        │êðèñòàëëè÷åñ- │       

                                                   │êèé                  

                                                   │íàòðèé        │8,0 ã  

                                                   │õëîðèñòûé            

                                                   │êàëèé         │1,6 ã  

                                                   │ôîñôîðíîêèñëûé│       

                                                   │îäíîçàìåùåííûé│       

                                                   │ïåïòîí 10%    │50,0 ìë │

                                                   │ðàñòâîð       │(êóá.  

                                                                 ñì)    

                                                   │äðîææåâîé     │22,0 ìë │

                                                   │ýêñòðàêò      │(êóá.  

                                                                 ñì)    

                                                   │áðèëëèàíòîâûé │5,0 ìë 

                                                   │çåëåíûé 0,1%  │(êóá.  

                                                   │âîäíûé ðàñòâîð│ñì)    

                      │Ñðåäà Ýíäî    │4,5 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,180 êã│

│8.  Áàêòåðèîëîãè÷åñêîå│ÌÏ àãàð       │2 ë (êóá. äì)  │ñóõîãî ïèòàò. │70,0 ã 

    │èññëåäîâàíèå                                   │àãàðà                

    │âîçäóõà           │Ñðåäà         │2,0 ë (êóá. äì)│ñóõîãî ïèòàò. │0,037 ã │

                      │×èñòîâè÷à                    │àãàðà                

                                                   │æåëòîê        │4 øò.  

                                                   │êóðèíîãî ÿéöà │       

                      │Êðîâÿíîé àãàð │2 ë (êóá. äì)  │ñóõîãî ïèòàò. │70,0 ã 

                                                   │àãàðà                

                                                   │äåôèáðèíèðî-  │100 ìë 

                                                   │âàííîé êðîâè         

                      │Ñðåäà Ýíäî    │20 ë (êóá. äì) │ñóõàÿ ñðåäà   │0,8 êã 

                      │Ñðåäà         │2,0 ë (êóá. äì)│-"-           │0,120 êã│

                      │Ïëîñêèðåâà                                        

                      │Âèñìóò-ñóëüôèò│2,0 ë (êóá. äì)│-"-           │0,120 êã│

                      │àãàð                                              

│9.  │Áàêèññëåäîâàíèå   │ÌÏÀ           │5,0 ë (êóá. äì)│ñóõîãî ïèòàò. │175,0 ã │

    │ìîëî÷íûõ ïèùåâûõ                               │àãàðà                

    │ïðîäóêòîâ è ïèâà. │Ñðåäà Êåññëåðà│3,0 ë (êóá. äì)│ïåïòîíà       │30,0 ã 

    │Îïðåäåëåíèå                                    │æåë÷è         │150 ìë 

    │êîëè-òèòðà è îáùåé│                                           │(êóá.  

    │îáñåìåíåííîñòè                                               ñì)    

                                                   │ëàêòîçû õ÷    │7,5 ã  

                                                   │ãåíöèàí-      │0,06 ã 

                                                   │ôèîëåòîâîãî          

                      │Ñðåäà Ýíäî    │3,0 ë (êóá. äì)│ñóõîé ñðåäû   │120 ã  

                      │Ñðåäà óãëåâîä.│500 ìë (êóá.   │ïåïòîíà       │5,0 ã  

                      │æèäêàÿ ñ      │ñì)            │NaCl          │2,5 ã  

                      │ïîïëàâêàìè è                 │óãëåâîäà      │5,0 ã  

                      │óãëåâîäîì                                         

                      │(ãëþêîçîé,                                        

                      │ëàêòîçîé)                                         

                      │Ñåëåíèòîâûé   │5,0 ë (êóá. äì)│ñåëåíèò       │20,0 ã 

                      │áóëüîí                       │íàòðèÿ NaHSeO │       

                                                                3│       

                      │Ñðåäà Ìþëëåðà │5,0 ë (êóá. äì)│ìåëà õ÷       │225,0 ã │

                                                   │á-íà          │4,5 ë  

                                                   │Õîòòèíãåðà    │(êóá.  

                                                                 │äì)    

                                                   │ð-ðà Ëþãîëÿ   │100 ìë 

                                                                 │(êóá.  

                                                                 ñì)    

                      │Ñðåäà         │2,0 ë (êóá. äì)│ñóõîé ñðåäû   │0,120 êã│

                      │Ïëîñêèðåâà                                        

                      Õëîðèñòî-ìàã- │5,0 ë (êóá. äì)│ñì. ïóíêò 3          

                      │íèåâàÿ ñðåäà                                      

                      │Ñðåäà Êîçåðà  │0,5 ë (êóá. äì)│              │16,0 ã 

│10. │Áàêèññëåäîâàíèå   │Ïèòàòåëüíûé   │3,0 ë (êóá. äì)│ñóõîãî ïèòàò. │150,0 ã │

    │ìÿñà è ìÿñíûõ     àãàð                         │àãàðà                

    │ïðîäóêòîâ         │Ñðåäà Êåññëåðà│6,5 ë (êóá. äì)│ïåïòîíà       │65,0 ã 

    │(êîëáàñíûå                                     │æåë÷è         │325,0 ìë│

    │èçäåëèÿ)                                                     │(êóá.  

                                                                 ñì)    

                                                   │ëàêòîçû õ÷    │16,25 ã │

                                                   │ãåíöèàí-âèîëåò│0,13 ã 

                      Ñåëåíèòîâàÿ   │5,0 ë (êóá. äì)│ñì. ïóíêò 3          

                      │ñðåäà                                             

                      │Ñðåäà Ìþëëåðà │5,0 ë (êóá. äì)│ñì. ïóíêò 9          

                      │Òðåõñàõàðíûé  │5,0 ë (êóá. äì)│ñì. ïóíêò 3          

                      │àãàð                                              

                      │Ñðåäà æèäêàÿ ñ│500,0 ìë (êóá. │ïåïòîíà       │5,0 ã  

                      │ïîïëàâêàìè è  ñì)            │NaCl          │2,5 ã  

                      │óãëåâîäàìè è                 │óãëåâîäà      │5,0 ã  

                      │ãëþêîçà,                                          

                      │ëàêòîçà,                                          

                      │ìàííèò,                                           

                      │ñàõàðîçà                                          

                      │Ñðåäà Õåéôåöà │0,5 ë (êóá. äì)│ñì. ïóíêò 4          

                      │Ñðåäà "ÕÁ"    │0,5 ë (êóá. äì)│ïåïòîíà       │5,0 ã  

                      │(õèíîçîëáðîì- │               │NaCl          │2,5 ã  

                      │êðåçîëïóðïóð-               │ìàííèò        │2,5 ã  

                      │íàÿ)                         │äðîææåâîé     │30,0 ìë │

                                                   │àâòîëèçàò     │(êóá.  

                                                                 ñì)    

                                                   │æåë÷ü         │15,0 ìë │

                                                   │êðóïíîãî      │(êóá.   

                                                   │ðîãàòîãî ñêîòà│ñì)    

                                                   │16% ñïèðòîâîãî│       

                                                   │ð-ðà áðîìêðå- │       

                                                   │çîëïóðïóðíîãî │       

                      │Ñðåäà Êîäà    │0,5 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,016 êã│

                      Õëîðèñòî-ìàã- │5,0 ë (êóá. äì)│ñì. ïóíêò 3          

                      │íèåâàÿ ñðåäà                                      

                      │Ñðåäà Ýíäî    │3,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,120 êã│

                      │Ñðåäà âèñìóò- │2,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,120 êã│

                      │ñóëüôèò àãàð                                      

                      │Ñðåäà         │2,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,120 êã│

                      │Ïëîñêèðåâà                                        

                      │Ñðåäà         │2,0 ë (êóá. äì)│ñóõîãî        │0,037 êã│

                      │×èñòîâè÷à                    │ïèòàòåëüíîãî         

                                                   │àãàðà                

                                                   │æåëòîê        │4 øò.  

                                                   │êóðèíîãî ÿéöà │       

                      │Ñðåäà Êëàðêà  │0,3 ë (êóá. äì)│àãàð-àãàð     │0,6 ã  

                                                   │ïåïòîí        │2,1 ã  

                                                   │ãëþêîçà       │1,5 ã  

                                                   │ôîñôîðíîêèñëûé│1,5 ã  

                                                   │êàäìèé               

                                                   │äâóçàìåùåííûé │       

│11. │Èññëåäîâàíèå      │ÌÏÀ (íà       │3,0 ë (êóá. äì)│ñóõîãî ïèòàò. │150,0 ã │

    │êóëèíàðíûõ èçäåëèé│ìèêðîáíîå                    │àãàðà                

    │èç ðóáëåíîãî ìÿñà │÷èñëî)                                            

                      │ÌÏÀ (íà       │0,5 ë (êóá. äì)│ñóõîãî ïèòàò. │25,0 ã 

                      │ïðîòåé)                      │àãàðà                

                      │Ñðåäà Ýíäî    │3,0 ë (êóá. äì)│ñóõîé ñðåäû   │120,0 ã │

                      │Ñåëåíèòîâûé   │5,0 ë (êóá. äì)│ñåëåíèò       │20,0 ã 

                      │áóëüîí                       │íàòðèÿ               

                      │Õëîðèñòî-     │5,0 ë (êóá. äì)│ìàãíèé        │195,0 ã │

                      │ìàãíèåâàÿ                    │õëîðèñòûé            

                      │ñðåäà (íà 500 │               │êðèñòàëëè÷åñ- │       

                      │ìë èññëåäóåìîé               │êèé                  

                      │æèäêîñòè)                    │NaCl          │40,0 ã 

                                                   │êàëèé         │8,0 ã  

                                                   │ôîñôîðíîêèñëûé│       

                                                   │îäíîçàìåùåííûé│       

                                                   │ïåïòîí 10% ð-ð│250,0 ìë│

                                                   │äðîææåâîé     │110,0 ìë│

                                                   │ýêñòðàêò             

                                                   │áðèëëèàíòîâûé │25,0 ìë │

                                                   │çåëåíûé 0,1%         

                                                   │âîäíûé ðàñòâîð│       

                      │Ñðåäà         │2,0 ë (êóá. äì)│ñóõîé ñðåäû   │0,120 ã │

                      │Ïëîñêèðåâà                                        

                      │Ñðåäà         │2,0 ë (êóá. äì)│ñì. ïóíêò 10         

                      │×èñòîâè÷à                                         

                      │Ñðåäà âèñìóò- │2,0 ë (êóá. äì)│ñì. ïóíêò 10         

                      │ñóëüôèò àãàð                                      

                      │Ñðåäà Êîäà    │0,5 ë (êóá. äì)│ñì. ïóíêò 10         

                      │Ñðåäà         │0,5 ë (êóá. äì)│ñì. ïóíêò 10         

                      │óãëåâîäíàÿ                                        

                      │æèäêàÿ ñ                                          

                      │ïîïëàâêàìè                                        

                      │(ãëþêîçà,                                         

                      │ëàêòîçà)                                          

                      │Ñðåäà Êåññëåðà│6,5 ë (êóá. äì)│ñì. ïóíêò 10         

│12. │Èññëåäîâàíèå      │ÌÏÀ           │3,0 ë (êóá. äì)│ñóõîé ñðåäû   │0,150 ã │

    │ðûáíîé êóëèíàðèè  │Ñðåäà Êåññëåðà│6,5 ë (êóá. äì)│èíãðåä. ñì.          

                                                   │ïóíêò 10             

                      │Ñðåäà Ýíäî    │3,0 ë (êóá. äì)│ñóõîé ñðåäû   │0,120 êã│

                      │Ñðåäà         │0,1 ë (êóá. äì)│ïåïòîíà       │10,0 ã 

                      óãëåâîäíàÿ                   │NaCl          │5,0 ã  

                      æèäêàÿ ñ                     │óãëåâîäà      │10,0 ã 

                      │ïîïëàâêàìè                                        

                      │(ãëþêîçà)                                         

                      │ÌÏÀ íà ïðîòåé │2,0 ë (êóá. äì)│ñóõîé ñðåäû   │0,1 êã 

                      │Ñðåäà Ìþëëåðà │5,0 ë (êóá. äì)│ìåëà õ÷       │225,0 ã │

                                                   │á-íà          │4,5 ë  

                                                   │Õîòòèíãåðà           

                                                   │ð-ð Ëþãîëÿ    │100,0 ìë│

                                                                 │(êóá.  

                                                                 ñì)    

                      │Âèñìóò-ñóëüôèò│2,0 ë (êóá. äì)│ñóõîé ñðåäû   │0,120 ã │

                      │àãàð                                              

                      │Ñðåäà         │2,0 ë (êóá. äì)│-"-           │0,120 ã │

                      │Ïëîñêèðåâà                                        

                      │Òðåõñàõàðíûé  │5,0 ë (êóá. äì)│ñîëü Ìîðà     │1,0 ã  

                      │àãàð (ñðåäà                  │ãèïîñóëüôèò   │1,5 ã  

                      │Îëüêåíèöêîãî) │               │íàòðèÿ ïåïòîí │0,1 êã 

                      │Ñðåäà Õåéôåöà │ñì. ïóíêò 4                         

                      │Ñðåäà Êîäà    │ñì. ïóíêò 10                        

                      │Ñðåäà "ÕÁ"    │ñì. ïóíêò 10                        

                      │Ñðåäà         │ñì. ïóíêò 10                        

                      │×èñòîâè÷à                                         

                      │Ñîëåâîé áóëüîí│0,5 ë (êóá. äì)│ïåïòîí        │5,0 ã  

                      │10%                          │NaCl          │32,5 ã 

│13. │Áàêèññëåäîâàíèå   │Ñðåäà Êåññëåðà│6,5 ë (êóá. äì)│ïåïòîíà              

    │íåñòàíäàðòíîé     │(íà êîëè-òèòð)│               │æåë÷è         │325,0 ìë│

    │ïðîäóêöèè (ñàëàòû,│                                           │(êóá.  

    │âèíåãðåòû) è                                                 │ñì)    

    │êóëèíàðíîé                                     │ëàêòîçû       │16,25 ã │

    │ïðîäóêöèè                                      │ãåíöèàí-      │0,13 ã 

                                                   │ôèîëåòîâîãî          

                      │ÌÏÀ (íà       │3,0 ë (êóá. äì)│ñóõîãî        │0,15 êã │

                      │ìèêðîáíîå                    │ïèòàòåëüíîãî         

                      │÷èñëî)                       │àãàðà                

                      Óãëåâîäíàÿ    │1,0 ë (êóá. äì)│ïåïòîíà       │10,0 ã 

                      │ñðåäà ñ                      │NaCl          │5,0 ã   

                      │ãëþêîçîé                     │óãëåâîäà      │10,0 ã 

                      │ÌÏÀ (íà       │2,0 ë (êóá. äì)│ñóõîé ïèòàò.  │0,1 êã 

                      │ïðîòåé)                      │àãàð                 

                      Ñåëåíèòîâàÿ   │5,0 ë (êóá. äì)│ñåëåíèò       │20,0 ã 

                      │ñðåäà                        │íàòðèÿ               

                      │Ñðåäà         │4,0 ë (êóá. äì)│àãàð-àãàð     │75 ã   

                      │×èñòîâè÷à                    │ïåïòîíà       │45 ã   

                                                   │NaCl          │31,5 ã 

                                                   │æåëòîê        │8 øò.  

                                                   │êóðèíîãî ÿéöà │       

                      │Ñðåäà Ýíäî    │3,0 ë (êóá. äì)│ñóõîé ñðåäû   │120,0 ã │

                      │Ñîëåâîé áóëüîí│0,5 ë (êóá. äì)│ïåïòîíà       │5,0 ã  

                      │10% (íà St.                  │NaCl          │325,0 ã │

                      │aureus)                                           

                      │Ñðåäà         │10,0 ë (êóá.   │ïèòàòåëüí.    │0,003 ã │

                      │Íèêîäåìóñà    │äì)            │àãàð                 

                      Õëîðèñòî-ìàã- │5,0 ë (êóá. äì)│ñì. ïóíêò 3          

                      │íèåâàÿ ñðåäà                                      

                      │Ñðåäà         │2,0 ë (êóá. äì)│ñì. ïóíêò 11         

                      │Ïëîñêèðåâà                                        

                      │Òðåõñàõàðíûé  │5,0 ë (êóá. äì)│ñì. ïóíêò 3          

                      │àãàð                                              

│14. │Áàêòåðèîëîãè÷åñêîå│Ñðåäà Êåññëåðà│6,5 ë (êóá. äì)│ïåïòîíà       │65,0 ã 

    │èññëåäîâàíèå ïî÷âû│                             │æåë÷è         │325,0 ìë│

                                                                 │(êóá.  

                                                                 ñì)    

                                                   │ëàêòîçà õ÷    │16,25 ã │

                                                   │ãåíöèàí-      │0,13 ã 

                                                   │ôèîëåòîâîãî          

                      │Ñðåäà Ýíäî    │10,0 ë (êóá.   │ñóõîé ñðåäû   │0,4 êã 

                                    │äì)                                 

                      │Óãëåâîäíàÿ    │500,0 ìë (êóá. │ïåïòîíà       │4,0 ã  

                      │ñðåäà ñ       │ñì)            │NaCl          │2,0 ã  

                      │ãëþêîçîé                     │óãëåâîäà      │4,0 ã  

                      │ÌÏÀ (íà       │5,0 ë (êóá. äì)│ñóõîé ïèòàò.  │175,0 ã │

                      │ìèêðîáíîå                    │àãàð                 

                      │÷èñëî)                                            

                      Ñåëåíèòîâàÿ   │5,0 ë (êóá. äì)│ñåëåíèò       │20,0 ã 

                      │ñðåäà                        │íàòðèÿ               

                      │Ñðåäà         │1,5 ë (êóá. äì)│ÌÏÀ           │1,5 ë  

                      │Âèëüñîí -                                  │(êóá.  

                      │Áëåðà                                      │äì)    

                                                   │ãëþêîçà       │15,0 ã 

                                                   │20% ð-ðà      │150 ìë 

                                                   │ñåðíèñòî-     │(êóá.  

                                                   │êèñëîãî íàòðèÿ│ñì)    

                                                   │8% ð-ð        │15,0 ã 

                                                   │õëîðèñòîãî           

                                                   │æåëåçà               

                      │Õëîðèñòî-     │5,0 ë (êóá. äì)│ñì. ïóíêò 3          

                      │ìàãíèåâàÿ                                         

                      │ñðåäà                                             

                      │Âèñìóò-ñóëüôèò│2,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │0,120 êã│

                      │àãàð                                              

                      │Òðåõñàõàðíûé  │0,5 ë (êóá. äì)│ñì. ïóíêò 6          

                      │àãàð                                              

│15. │Áàêèññëåäîâàíèå   │Ãëþêîçî-      │1,7 ë (êóá. äì)│ïåïòîí        │0,15 ã 

    │ëåêàðñòâåííûõ ôîðì│ïåïòîííàÿ                    ôåðìåíòàòèâíûé       

    │èç àïòåê          │ñðåäà                                             

                      │Ñðåäà Ýíäî    │0,4 ë (êóá. äì)│ñóõàÿ ñðåäà   │16,0 ã 

                                                   │Ýíäî                 

                      │ÌÏÎ íà ìèêðîá.│2,4 ë (êóá. äì)│ñóõîé ïèòàò.  │2,2 ã  

                      │÷èñëî                        │àãàð                 

                      │ÌÏÀ íà ïðîòåé │0,8 ë (êóá. äì)│-"-           │0,8 ã  

                      │ÌÏÀ íà        │1,0 ë (êóá. äì)│-"-           │1,0 ã  

                      │ïëåñíåâ.                                          

                      │ãðèáêè                                            

                      │(Ñàáóðî)                                          

                      │Ñðåäà Êåññëåðà│0,5 ë (êóá. äì)│ñì. ïóíêò 4          

                      │Ñîëåâîé áóëüîí│0,5 ë (êóá. äì)│ñì. ïóíêò 13         

                      │10%                                               

                      │Ñðåäà         │0,4 ë (êóá. äì)│ñì. ïóíêò 5          

                      │×èñòîâè÷à                                         

                      │Àãàð          │0,3 ë (êóá. äì)│ôåíèëàëàíèí   │0,3 ã  

                      │ôåíèëàëàíèíî- │                                    

                      │âûé                                               

│16. │Èññëåäîâàíèå      │ÌÏÁ           │12,0 ë (êóá.   │áóëüîí ñòàíä. │12,0 ë 

    │õèðóðãè÷åñêîãî                  │äì)                          │(êóá.  

    │ìàòåðèàëà íà                                                 │äì)    

    │ñòåðèëüíîñòü      │Ïåïòîí                       │ïåïòîí ñóõîé  │120,0 ã │

                      │Ñðåäà Ñàáóðî  │1,0 ë (êóá. äì)│ïåïòîí        │200,0 ã │

                                                   │ôåðìåíòàòèâíûé│       

                      │Òèîãëèêîëåâàÿ │2,0 ë (êóá. äì)│ñóõàÿ ñðåäà   │60,0 ã 

                      │ñðåäà                                             

                      │Áóëüîí        │1,0 ë (êóá. äì)│ãëþêîçà       │5,0 ã  

                      │Õîòòèíãåðà ñ                 │NaCl          │5,0 ã  

                      │0,5% ãëþêîçîé │                                    

                      │Ñðåäà         │1,5 ë (êóá. äì)│(íà 100              

                      │×èñòîâè÷à                    │âûäåëåííûõ           

                                                   │êóëüòóð)             

                      │Ñðåäà Ýíäî    │0,5 ë (êóá. äì)│ñì. ïóíêò 4          

                      │Ñîëåâîé áóëüîí│0,5 ë (êóá. äì)│ñì. ïóíêò 13         

│17. │Èññëåäîâàíèå      │Ñàõàðíûé      │1,3 ë (êóá. äì)│ãëþêîçà       │13,0 ã 

    │êîíñåðâîâ íà      │áóëüîí                                            

    │ñòåðèëüíîñòü      │Ñðåäà         │1,5 ë (êóá. äì)│ñì. ïóíêò 14         

                      │Âèëüñîí - Áëåð│                                    

└────┴──────────────────┴──────────────┴───────────────┴──────────────┴────────┘

 

Êîëè÷åñòâî èíãðåäèåíòîâ ðÿäà ðåàêòèâîâ,

èíäèêàòîðîâ è êðàñîê

 

┌────┬──────────────┬─────────────────┬──────────────────────┬─────────────────┐

│ N  │ Íàèìåíîâàíèå │      Îáùåå           Èíãðåäèåíòû         Êîëè÷åñòâî   

ï/ï │   ðåàêòèâà,       êîë-âî                              èíãðåäèåíòîâ  

      èíäèêàòîðà                                                         

├────┼──────────────┼─────────────────┼──────────────────────┼─────────────────┤

│ 1        2               3                  4                   5       

├────┼──────────────┼─────────────────┼──────────────────────┼─────────────────┤

│1.  │Ðåàêòèâ Ýðëèõà│90 ìë (êóá. ñì)  │ïàðàäèìåòèëàìèäî-     │0,9 ã           

                                   │áåíçàëüäåãèä                          

                                   │ñïèðò 96°             │90,0 ã          

                                   │ñîëÿíàÿ êèñëîòà       │9,0 ã           

                                   │êîíöåíòðèðîâàííàÿ                     

│2.  │Ðåàêòèâ Êîâà÷à│210 ìë (êóá. ñì) │àìèëîâûé ñïèðò        │150 ìë (êóá. ñì) │

                                   │ñîëÿíàÿ êèñëîòà êîíö. │50,0 ìë         

                                   │ïàðàäèìåòèëàìèäî-     │10,2 ã          

                                   │áåíçàëüäåãèä                           

│3.  │Îêðàñêà ïî                     ãåíöèàí-ôèîëåòîâîãî   │0,25 ã          

    │Ãðàìó                                                                

    │à) ðàñòâîð äëÿ│25 ìë (êóá. ñì)  │à) ñïèðòà 96°         │25 ã            

    │ïðèãîòîâëåíèÿ │                 │ãëèöåðèíà             │1,25 ã          

    │áóìàæåê Ñèíåâà│                                                       

    │á) ðàñòâîð    │60 ìë (êóá. ñì)  │á) éîäà êðèñòàëëè÷.   │0,2 ã           

    │Ëþãîëÿ                         │éîäèñòîãî êàëèÿ       │0,4 ã           

    │â) ñïèðòà 96° │150 ìë (êóá. ñì) │â) ñïèðòà 96°         │150,0 ìë (êóá.  

    │äëÿ                                                  ñì)             

    │îáåñöâå÷èâàíèÿ│                                                       

    │ã) ôóêñèíà    │10 ìë            │ã) îñíîâíîãî ôóêñèíà  │0,1 ã           

    │Öèëÿ                           │êàðáîëîâîé êèñëîòû                    

                                   │êîíöåíòðèðîâàí.                       

                                   │ãëèöåðèíà             │0,05 ã          

                                   │ñïèðòà 96°            │1,0 ã           

│4.  │Ìåòèëåíîâûé   │300 ìë (êóá. ñì) │ìåòèëåíîâîãî ñèíåãî   │0,9 ã           

    │ñèíèé ïî                       ñïèðòà 96°            │90,0 ìë (êóá. ñì)│

    │Ëåôôëåðó                       │KOH                   │0,03 ã          

│5.  │Èíäèêàòîð     │100 ìë (êóá. ñì) │êèñëûé ôóêñèí         │0,5 ã           

    │Àíäðåäå                        │NaOH 4% ðàñòâîð       │16,4 ìë (êóá. ñì)│

│6.  │Îêðàñêà ïî                                                           

    │ìåòîäó Íåéñåðà│                                                        

    │à) óêñóñíîêèñ-│100 ìë (êóá. ñì) │à) ìåòèëåíîâàÿ ñèíÿÿ  │0,1 ã           

    │ëàÿ ñèíÿÿ                      │ñïèðò 96°             │2,0 ìë (êóá. ñì) │

    │Íåéññåðà                       │óêñóñíàÿ êèñëîòà      │5,0 ìë (êóá. ñì) │

                                   │ëåäÿíàÿ                               

                                   │äèñòèëëèðîâ. âîäà     │100 ìë (êóá. ñì) │

    │á) âåçóâèí    │100 ìë (êóá. ñì) │á) âåçóâèí            │2,0 ã           

                                   │ñïèðò 96°             │60,0 ìë (êóá. ñì)│

                                   │äèñòèëëèðîâ. âîäà     │40,0 ìë (êóá. ñì)│

    │â) ðàñòâîð    │300 ìë (êóá. ñì) │â) éîäà êðèñòàëëè÷.   │1,0 ã           

    │Ëþãîëÿ                         │éîäèñòîãî êàëèÿ       │2,0 ã           

                                   │äèñòèëëèðîâ. âîäû äî  │300 ìë (êóá. ñì) │

│7.  │Îêðàñêà ïî                                                           

    │ìåòîäó Öèëÿ - │                                                       

    │Íèëüñåíà                                                             

    │à) êàðáîëîâûé │116 ìë (êóá. ñì) │à) ôóêñèí (îñíîâíîé)  │1,0 ã           

    │ôóêñèí                         │êàðáîëîâàÿ êèñëîòà    │5,0 ã           

                                   │êðèñòàëëè÷åñêàÿ                       

                                   │ñïèðò 96°             │10,0 ìë (êóá. ñì)│

                                   │ãëèöåðèí              │êàïëè           

                                   │âîäà äèñòèëëèð.       │100,0 ìë (êóá.  

                                                         ñì)             

    │á) ñåðíàÿ                      │á) ñåðíàÿ êèñëîòà 5%                   

    │êèñëîòà                                                              

    │â) ìåòèëåíîâàÿ│110 ìë (êóá. ñì) │â) ìåòèëåíîâàÿ ñèíÿÿ  │10,0 ã          

    │ñèíÿÿ                          │ñïèðò 96°             │100,0 ìë (êóá.  

                                                         ñì)             

│8.  │Ðåàêòèâ äëÿ   │110 ìë (êóá. ñì) │Ðåàêòèâ A                             

    │îïðåäåëåíèÿ                    │ìî÷åâèíà              │2,0 ã           

    │óðåàçû (ïî                     │96° ýòèëîâûé ñïèðò    │2,0 ìë (êóá. ñì) │

    │ìåòîäó Çàêñà) │                 │äèñòèëëèð. âîäà       │4,0 ìë (êóá. ñì) │

                                   │Ðåàêòèâ B                             

                                   │0,2% ð-ð ôåíîëðîò     │1,0 ìë (êóá. ñì) │

                                   │îäíîçàìåùåííûé ôîñôàò │0,1 ã           

                                   │êàëèÿ (KH PO )                        

                                            2  4                         

                                   │äâóçàìåùåííûé ôîñôàò  │0,1 ã           

                                   │êàëèÿ (KH PO )                        

                                            2  4                         

                                   │õëîðèñòûé íàòðèé      │0,5 ã           

                                   │(NaCl)                                

                                   │äèñòèëëèðîâ. âîäà     │100 ìë (êóá. ñì) │

│9.  │Ðåàêòèâ äëÿ                    │Ðàñòâîð N 1                           

    │îïðåäåëåíèÿ                    │1% ñïèðòîâîé ð-ð                      

    │îêñèäàçíîé                     àëüôà-íàôòîëà                         

    │àêòèâíîñòè                     │Ðàñòâîð N 2                           

    │áàêòåðèé                       │1% âîäíûé ð-ð äèìåòèë-│                

                                   │ï-ôåíèëåíäèàìèíà                      

                                   │Ãîòîâÿò ñìåñü ïåðåä   │3 ÷àñòè N 1     

                                   │óïîòðåáëåíèåì         │7 ÷àñòåé N 2    

│10. │Óêñóñíîêèñëàÿ │100 ìë (êóá. ñì) │òîëóèäèíîâàÿ ñèíü     │0,25 - 0,5 ã    

    │òîëóèäèíîâàÿ                   ëåäÿíàÿ óêñóñíàÿ      │2,0 ìë (êóá. ñì) │

    │ñèíü                           │êèñëîòà                               

                                   │äèñòèëëèðîâ. âîäà äî  │100 ìë (êóá. ñì) │

│11. │Ìåòèë-âèîëåò  │100 ìë (êóá. ñì) │ìåòèë-âèîëåò èëè      │0,25 ã          

    │èëè êðèñòàëë-                 │êðèñòàëë-âèîëåò                       

    │âèîëåò                         │5% óêñóñíàÿ êèñëîòà   │100,0 ìë (êóá.  

                                                         ñì)             

└────┴──────────────┴─────────────────┴──────────────────────┴─────────────────┘

 

 

 

 

 

Ïðèëîæåíèå 1

 

ÏÅÐÅ×ÅÍÜ

ÏÈÒÀÒÅËÜÍÛÕ ÑÐÅÄ ÏÐÎÌÛØËÅÍÍÎÃÎ ÈÇÃÎÒÎÂËÅÍÈß

 

┌────┬───────────────────────────────┬────────────────────┬────────────────────┐

│ N        Íàèìåíîâàíèå ñðåäû       │Ïðîèçâîäñò. èíñòèòóò│Íîðìàòèâû, äîêóìåíòû│

ï/ï │                                                        (ÌÐÒÓ, ÒÓ)    

├────┼───────────────────────────────┼────────────────────┼────────────────────┤

│ 1                2                         3                   4         

├────┼───────────────────────────────┼────────────────────┼────────────────────┤

│1.  │Áàêòîàãàð Ïëîñêèðåâà ñóõîé     Äàãåñòàíñêèé ÍÈÈÏÑ  │ÒÓ 42 N 326-82     

│2.  │Àãàð Ýíäî ñóõîé                │Äàãåñòàíñêèé ÍÈÈÏÑ  │ÒÓ 42.14 N 96-77   

│3.  │Àãàð ñ ýîçèí-ìåòèëåíîâûì ñèíèì │-"-                 │ÒÓ 42.14 N 95-77   

    │(Ëåâèíà)                                                              

│4.  Ïèòàòåëüíûé àãàð ñóõîé         │-"-                 │ÒÓ 42.14 N 33-75   

│5.  │Âèñìóò-ñóëüôèò àãàð ñóõîé      -"-                 │ÒÓ 42.14 N 127-78  

│6.  │Ñðåäà ñ èíäèêàòîðîì ÂÐ è îäíèì │Äàãåñòàíñêèé ÍÈÈÏÑ  │ÒÓ 42.14 N 63-76   

    │èç óãëåâîäîâ (ãëþêîçîé,                                               

    │ëàêòîçîé, ìàëüòîçîé, ñàõàðîçîé)│                                       

    │èëè ìíîãîàòîìíûì ñïèðòîì                                              

    │ìàííèòîì                                                               

│7.  │Ïèòàòåëüíàÿ ñðåäà Ðåññåëÿ ñóõàÿ│Äàãåñòàíñêèé ÍÈÈÏÑ  │ÒÓ 42.14 N 128-78  

│8.  │Ïèòàòåëüíûé áóëüîí             │-"-                                    

│9.  │Ïèòàòåëüíàÿ ñðåäà äëÿ          Ìîñêîâñêèé ÈÂÑ      │ÒÓ 42.14 N 159-79  

    │ïåðâè÷íîé äèôôåðåíöèàöèè       │èì. Ìå÷íèêîâà                          

    │ýíòåðîáàêòåðèé ñóõàÿ (òèïà                                            

    │Êëèãëåðà)                                                             

│10. │Ïèòàòåëüíàÿ ñðåäà Êîäà         Äàãåñòàíñêèé ÍÈÈÏÑ  │ÒÓ 42.14 N 2-74    

│11. │Ïèòàòåëüíàÿ ñðåäà ñóõàÿ,       Äàãåñòàíñêèé ÍÈÈÏÑ  │ÒÓ 42.14 N 154-79  

    ïðåäíàçíà÷åííàÿ äëÿ îïðåäåëåíèÿ│                                       

    │Â-ãàëàêäîçèäàçû ýíòåðîáàêòåðèè │                                       

│12. │Àãàð ùåëî÷íîé ñóõîé            Ìîñêîâñêèé ÍÈÈÂèÑ   │ÒÓ 42.14 N 84-76   

                                   │èì. Ìå÷íèêîâà                          

│13. │Àãàð ùåëî÷íîé äðîææåâîé ñóõîé  │Ðîñòîâ-íà-Äîíó, Ï×È │ÒÓ 42              

                                                       │ÊÂÑ 90-76          

│14. │Âèòàìèííûé ïðåïàðàò (ÝÊÄ) ñóõîé│Ìîñêîâñêèé ÍÈÈÂèÑ   │ÒÓ 42.14 N 56-76   

    │äëÿ ìèêðîáèîëîãè÷åñêèõ         │èì. Ìå÷íèêîâà                          

    │ïèòàòåëüíûõ ñðåä                                                      

│15. │Ïåïòîí îñíîâíîé ñóõîé          -"-                 │ÒÓ 42.14 N 85-76   

│16. │Êàçåèíîâî-óãîëüíûé àãàð ñóõîé  │Äàãåñòàíñêèé ÍÈÈÏÑ  │ÒÓ 42.14 N 111-77  

    │(ÊÓÀ)                                                                 

│17. │Ýëåêòèâíàÿ õèíîçîëüíàÿ         │-"-                 │ÒÓ 42.14 N 126-78  

    │èíäèêàòîðíàÿ ïèòàòåëüíàÿ ñðåäà │                                        

    │äëÿ ìèêðîáèîëîãè÷åñêîé                                                

    │äèàãíîñòèêè äèôòåðèè (ñðåäà                                           

    │Áó÷èíà)                                                               

│18. │Ïèòàòåëüíûé àãàð äëÿ           │-"-                 │ÒÓ 42.14 N 105-77  

    │îïðåäåëåíèÿ òîêñèãåííîñòè                                             

    │äèôòåðèéíûõ ìèêðîáîâ ñóõîé                                            

    │(ÎÒÄÌ)                                                                

│19. │Òèàìèí-öèñòèí-ãëóòàìèíîâûé àãàð│-"-                 │ÒÓ 42.14 N 152-79  

    │ñóõîé                                                                 

│20. │Àãàð ýëåêòèâíûé ñîëåâîé äëÿ    │Ìîñêîâñêèé ÍÈÈÂèÑ   │ÒÓ 42.14 N 156-79  

    │ñòàôèëîêîêêîâ                  │èì. Ìå÷íèêîâà                          

│21. │Ñðåäà äëÿ êîíòðîëÿ ñòåðèëüíîñòè│-"-                 │ÒÓ 42.14 N 161-79  

    │(òèîãëèêîëåâàÿ ñðåäà)                                                 

│22. │Ïèòàòåëüíàÿ ñðåäà äëÿ          Äàãåñòàíñêèé ÍÈÈÏÑ  │ÒÓ 42.14 N 129-78  

    │îïðåäåëåíèÿ ÷óâñòâèòåëüíîñòè                                          

    │ìèêðîîðãàíèçìîâ ê àíòèáèîòèêàì │                                       

    │ñóõàÿ (ÀÃÂ)                                                           

│23. │Ñðåäà äëÿ îïðåäåëåíèÿ          -"-                                    

    │àêòèâíîñòè àíòèáèîòèêîâ N 8                                           

│24. │Ñèñòåìû èíäèêàòîðíûå áóìàæíûå  Ãîðüêîâñêèé ÈÝÌ     │ÒÓ 42.14 N 123-78  

    │(ÑÈÁ) äëÿ óñêîðåííîé                                                  

    │èäåíòèôèêàöèè âèáðèîíîâ                                                

│25. │Ñèñòåìû èíäèêàòîðíûå áóìàæíûå  Ãîðüêîâñêèé ÈÝÌ     │ÒÓ 42.14 N 128-78  

    │(ÑÈÁ) äëÿ ïîñòàíîâêè                                                  

    │îêñèäàçíîãî òåñòà è ôåðìåíòàöèè│                                        

    │ãëþêîçû                                                               

│26. │Ñóõîé ôåðìåíòàòèâíûé ãèäðîëèçàò│Ìîñêîâñêèé ÍÈÈÂèÑ   │ÒÓ 42.14 N 1-74    

    │êàíèíà íåãëóáîêîé ñòåïåíè      │èì. Ìå÷íèêîâà                          

    │ðàñùåïëåíèÿ                                                           

│27. │Êèñëîòíûé ãèäðîëèçàò êàçåèíà   │Äàãåñòàíñêèé ÍÈÈÏÑ  │ÒÓ 42.14 N 108-78  

    │ñðåäíåé ñòåïåíè ðàñùåïëåíèÿ                                           

│28. │Ìÿñîïåïòîííûé áóëüîí           │Áåëîðóññêèé         │ÒÓ 42.14 N 270-82  

│29. │Ìÿñîïåïòîííûé àãàð             │Áåëîðóññêèé         │ÒÓ 42.14 N 271-82  

│30. │Ïèòàòåëüíûé áóëüîí ñóõîé       │Äàãåñòàíñêèé ÍÈÈÏÑ  │ÒÓ 42.14 N 83-76   

└────┴───────────────────────────────┴────────────────────┴────────────────────┘

 

 

 

 

 

Ïðèëîæåíèå 2

 

ÏÅÐÅ×ÅÍÜ

ÏÈÒÀÒÅËÜÍÛÕ ÑÐÅÄ ËÀÁÎÐÀÒÎÐÍÎÃÎ ÈÇÃÎÒÎÂËÅÍÈß

 

┌────┬──────────────────────┬──────────────────────────────┬───────────────────┐

│ N       Íàèìåíîâàíèå              Èíãðåäèåíòû          │Êîë-âî èíãðåäèåíòîâ│

│ï/ï │        ñðåäû                     ñðåäû             │ íà 1 ë (êóá. äì) 

                                                           ãîòîâîé ñðåäû  

├────┼──────────────────────┼──────────────────────────────┼───────────────────┤

│ 1            2                         3                        4        

├────┼──────────────────────┼──────────────────────────────┼───────────────────┤

│1.  │Òðåõñàõàðíûé àãàð ñ   ëàêòîçà                       │10,0 ã            

    │ìî÷åâèíîé             │ñîëü Ìîðà                     │0,2 ã             

    │(È.Ñ. Îëüêåíèöêîãî)   │ãèïîñóëüôèò                   │0,3 ã             

                          │ñàõàðîçà                      │10,0 ã             

                          │ãëþêîçà                       │10,0 ã            

                          │ìî÷åâèíà                      │10,0 ã            

                          │ð-ð ôåíîëîâîãî êðàñíîãî       │4,0 ìë (êóá. ñì)  

│2.  │Ñðåäû óãëåâîäíûå      │ïåïòîíà                       │10,0 ã            

    │æèäêèå ñ ïîïëàâêîì    │õëîðèñòîãî íàòðèÿ             │5,0 ã             

    │(êñèëîçà, àðàáèíîçà,  │óãëåâîäà (êàæäîãî)            │10,0 ã            

    │äóëüöèò è ò.ä.)       │ôóêñèí êèñëîãî                │0,05 ã            

                          │IN p-pa NaOH                  │1,6 ã             

│3.  Ãëèöåðèíîâûé àãàð     │ñóõîãî ïèòàòåëüí. àãàðà       │18,0 ã            

                          │ãëèöåðèíà                     │10,0 ã            

                          │ôóêñèíà êèñëîãî               │10,05 ã           

                          │IN p-pa NaOH                  │1,6 ã             

│4.  │Ïåïòîííàÿ âîäà 1% äëÿ │ïåïòîíà                       │10,0 ã            

    │îïðåäåëåíèÿ èíäîëà,   │õëîðèñòîãî íàòðèÿ             │5,0 ã             

    │ñåðîâîäîðîäà                                                          

│5.  │Ïîëóæèäêèé àãàð 0,2%  │àãàð-àãàðà                    │2,0 ã             

    │äëÿ îïðåäåëåíèÿ       │ïåïòîíà                       │10,0 ã            

    │ïîäâèæíîñòè           │õëîðèñòîãî íàòðèÿ             │5,0 ã             

│6.  Ïèòàòåëüíûé àãàð      │ñóõîãî ïèòàòåëüíîãî àãàðà     │50,0 ã            

│7.  │Ñðåäà Êðèñòîíñåíà     │äðîææåâîãî ýêñòðàêòà          │22,0 ìë (êóá. ñì) 

                          │ëèìîííîêèñëîãî íàòðèÿ         │3,0 ã             

                          │2Na C (H O  x 11H O)                             

                             2 6  5 7      2                              

                          │ãëþêîçû õ÷                    │0,2 ã             

                          │öèñòèíà ñîëÿíîêèñëîãî         │0,1 ã             

                          │îäíîçàìåùåííîãî (KH PO )      │1,0 ã             

                                             2  4                         

                          │ôîñôîðíîêèñëîãî êàëèÿ                           

                          │õëîðèñòîãî íàòðèÿ             │5,0 ã             

                          │èíäèêàòîðà ôåíîëîâîãî êðàñíîãî│3,0 ìë (êóá. ñì)  

                          │0,4% âîäíîãî ðàñòâîðà                           

                          │àãàð-àãàðà                    │15,0 ìë (êóá. ñì) 

                          │äèñòèëëèðîâàííàÿ âîäà         │1000,0 ìë (êóá. ñì)│

│8.  │Ñðåäà îáîãàùåíèÿ ñ    │ñåëåíèòà íàòðèÿ (NaHSeO )     │4,0 ã             

    │ñåëåíèòîì íàòðèÿ                             3                        

                          │Na HPO                        │7,0 ã             

                            2   4                                         

                          │NaH PO                        │3,0 ã             

                             2  4                                         

                          │ïåïòîíà                       │5,0 ã             

                          │ëàêòîçû õ÷                    │4,0 ã             

│9.  │Ñðåäà îáîãàùåíèÿ      │ìåëà õ÷                       │45,0 ã            

    │Ìþëëåðà               │áóëüîíà Õîòòèíãåðà            │900,0 ìë (êóá. ñì) │

                          │ðàñòâîðà Ëþãîëÿ â òîì ÷èñëå:  │20,0 ìë (êóá. ñì) 

                          │éîäèñòîãî êàëèÿ               │4,0 ã             

                          │éîäà êðèñòàëëè÷åñêîãî         │5,0 ã             

                          │ãèïîñóëüôèòà                  │50,0 ã             

│10. │Ñðåäà Êëàóáåðãà II    │ñóõîãî ïèòàòåëüíîãî àãàðà     │75,0 ã            

                          │èëè àãàð-àãàðà                │18,0 ã            

                          │ãëèöåðèíà õ÷                  │3,3 ã             

                          │äåôèáðèíèðîâàííîé êðîâè       │5,9 ìë (êóá. ñì)  

                          │2% ð-ðà òåëëóðèòà êàëèÿ       │18,0 ã            

│11. │Êðîâÿíîé òåëëóðèòîâûé │ñóõîãî ïèòàòåëüíîãî àãàðà     │75,0 ã            

    │àãàð                  │èëè àãàð-àãàðà                │18,0 ã            

                          │äåôèáðèíèðîâàííîé êðîâè       │100,0 ìë (êóá. ñì) │

                          │2% ð-ðà òåëëóðèòà êàëèÿ       │10,0 ìë (êóá. ñì) 

│12. │Öèñòèí-òåëëóðèò       │ñóõîãî ïèòàò. àãàðà           │50,0 ã            

    │ñûâîðîòî÷íàÿ ñðåäà    │1% ð-ðà öèñòèíà â 0,1 N ð-ðå  │120,0 ìë (êóá. ñì) │

    │(ñðåäà Òèíñäàëÿ â     │NaOH                                            

    │ìîäèôèêàöèè Ñàäûêîâîé)│â òîì ÷èñëå öèñòèí            │1,2 ìë (êóá. ñì)  

                          │2,5% ð-ðà ãèïîñóëüôèòà íàòðèÿ │18,0 ìë (êóá. ñì) 

                          │â òîì ÷èñëå ïîðîøêà           │0,45 ìë (êóá. ñì) 

                          │ãèïîñóëüôèòà íàòðèÿ                             

                          │2% ð-ðà òåëëóðèòà êàëèÿ       │18,0 ìë (êóá. ñì) 

                          │íàòèâíîé ñûâîðîòêè êð. óáèòîãî│200,0 ìë (êóá. ñì) │

                          │ñêîòà                                           

│13. │Ñðåäû óãëåâîäíûå ñ    │ïåïòîíà                       │2,4 ã             

    │ãëþêîçîé, ñàõàðîçîé,  │NaCl                          │1,2 ã             

    │êðàõìàëîì             │ãëþêîçû èëè ñàõàðîçû          │1,2 ã              

                          │êðàõìàëà ðàñòâîðèìîãî         │0,48 ã            

                          │ôóêñèíà êèñëîãî               │0,02 ã            

                          │IN ð-ðà NaOH                  │0,39 ã            

│14. │Ñðåäà Ëåôôëåðà        │ìåòèëåíîâîãî ñèíåãî           │9,0 ã             

                          │ñïèðòà                        │300,0 ã           

                          │KOH                           │0,1 ã             

│15. │Ñðåäà Ïèçó            │2% ÌÏÀ                        │216 ìë (êóá. ñì)  

                          │1% ð-ð öèñòèíà â 0,1 N ð-ðå   │48 ìë (êóá. ñì)   

                          │NaOH                                            

                          │â òîì ÷èñëå öèñòèíà           │0,05 ã            

                          │óêñóñíîêèñëîãî ñâèíöà         │0,24 ã            

                          │íîðìàëüíîé ëîøàäèíîé ñûâîðîòêè│21,6 ìë (êóá. ñì) 

│16. │Áóëüîí ñ ìî÷åâèíîé    │ÌÏÁ                           │100 ìë (êóá. ñì)  

                          │ìî÷åâèíà                      │10,0 ã            

                          │êðåçîëîâîãî êðàñíîãî          │0,016 ã           

│17. │Ñîëåâîé áóëüîí 5%     │ïåïòîíà                       │10,0 ã            

                          │NaCl                          │5,0 ã             

│18. │Ñàõàðíûé áóëüîí       │ìÿñíîé âîäû                   │1000,0 ìë (êóá. ñì)│

                          │ïåïòîíà                       │10,0 ã             

                          │NaCl                          │5,0 ã             

                          │ãëþêîçû                       │10,0 ã            

│19. │Ñðåäà Ðàïîïîðò        │æåë÷è                         │10,0 ìë (êóá. ñì) 

                         │ìÿñíîãî áóëüîíà               │90,0 ìë (êóá. ñì) 

                          │ãëþêîçû õ÷                    │20,0 ã            

                          │êèñëîãî ôóêñèíà               │0,05 ã            

                          │IN ð-ðà NaOH                  │1,6 ìë (êóá. ñì)  

│20. │Ñðåäà ×èñòîâè÷à       │àãàð-àãàð                     │18,76 ã           

                          │ïåïòîíà                       │11,25 ã           

                          │NaCl                          │78,75 ã           

                          │æåëòîê êóðèíîãî ÿéöà          │2 øò.             

│21. │Ãëþêîçîïåïòîííàÿ ñðåäà│ïåïòîíà                       │100 ã             

                          │NaCl                          │50,0 ã            

                          │ãëþêîçû                       │50,0 ã            

│22. │Ñðåäà Êåññëåðà        │ïåïòîí                        │10,0 ã            

    │(ÃÎÑÒ 9225-68 "Ìîëîêî │æåë÷ü                         │50,0 ìë (êóá. ñì) 

    │è ìîëî÷íûå ïðîäóêòû") │ãëþêîçà                       │2,5 ìë (êóá. ñì)  

                          │1% âîäíûé ðàñòâîð             │2,0 ìë (êóá. ñì)  

                          ãåíöèàí-ôèîëåòîâûé                              

│23. │Ñðåäà Ñèìîíñà         │ôîñôîðíîêèñëûé íàòðèé àììîíèé │15,0 ã            

                          │îäíîêèñëûé ôîñôîðíîêèñëûé     │1,0 ã             

                          │êàëèé                                            

                          │ñåðíîêèñëûé ìàãíèé            │0,2 ã             

                          │íàòóðàëüíûé ëèìîííîêèñëûé     │3,0 ã             

                          │íàòðèé                                          

                          │àãàð-àãàð                     │20,0 ã            

                          │áðîìòèìîëîâîãî ñèíåãî         │0,016 ã           

│24. │Ñðåäà "6-Á"           │àìèíîïåïòèä                   │250,0 ìë (êóá. ñì) │

                          │ìÿñíàÿ âîäà                   │750,0 ìë (êóá. ñì) │

                          │äðîææåâîé àóòîëèçàò           │10,0 ìë (êóá. ñì) 

                          │ãëþêîçà                       │5,0 ã             

                          │àãàð-àãàð                     │1,0 ã             

│25. │Øîêîëàäíûé àãàð       │ïèòàòåëüíûé àãàð              │75,0 ã            

    │Ëåâèíòàëÿ             │10% äåôèáðèíèðîâàííàÿ êðîâü   │100,0 ìë (êóá. ñì) │

│26. │Ñðåäà "ÖÏÕ" (Ìîðîç    │1,5 - 2% ÌÏÀ                                    

    │À.Ô., Àôèíîãåíîâ Ã.Å.,│Äàãåñòàíñêèé ïåïòîí           │20,0 ã            

    │1976)                 │êàëèé îäíîêèñëûé              │10,0 ã            

                          │ìàãíèé õëîðèñòûé              │1,5 ã             

                          │Ì-öåòèëïèðèäèíîâûé õëîðèä     │2,0 ã             

                          │âîäà äèñòèëëèðîâàííàÿ         │1000,0 ìë (êóá. ñì)│

│27. │Ñðåäà "ÑÏÏ" (Ìîðîç    │1% ÌÏÀ                                          

    │À.Ô., Àôèíîãåíîâ Ã.Å.,│Äàãåñòàíñêèé ïåïòîí           │2%                

    │1978)                 │ñ äðîææåâûì ýêñòðàêòîì                          

                          │ëèìîííîêèñëûé íàòðèé          │10%                

                          │ãëþêîçà                       │0,125%            

                          │êàëèé ñåðíîêèñëûé             │1%                

                          │àãàð-àãàð                     │1,2%              

│28. │Ñðåäà "Ê-1" (Êàëèíà   │âîäà äèñòèëëèðîâàííàÿ         │1000,0 ìë (êóá. ñì)│

    │Ã.Ï., 1980)           │ñóëüôàò ìàãíèÿ                │0,2 ã             

                          │õëîðèä íàòðèÿ                 │5,0 ã             

                          │äâóçàìåùåííûé ôîñôîðíîêèñëûé  │2,0 ã             

                          │êàëèé                                           

                          │îäíîçàìåùåííûé ôîñôîðíîêèñëûé │2,0 ã             

                          │êàëèé                                           

                          │ðàôèíîçà                      │2,0 ã             

│29. │Ñðåäà "Ê-2" (Êàëèíà   │âîäà äèñòèëëèðîâàííàÿ         │1000,0 ìë (êóá. ñì)│

    │Ã.Ï., 1980)           │ñóëüôàò ìàãíèÿ                │0,2 ã             

                          │õëîðèä íàòðèÿ                 │5,0 ã             

                          │äâóçàìåùåííûé ôîñôîðíîêèñëûé  │2,0 ã             

                          │êàëèé                                           

                          │îäíîçàìåùåííûé ôîñôîðíîêèñëûé │0,8 ã             

                          │êàëèé                                           

                          │ðàôèíîçà                      │5,0 - 10,0 ã      

                          │àãàð                          │20,0 ã            

                          │ñåðíîêèñëûé êàëèé             │0,2 ã             

│30. │Ñðåäà "À-1" æèäêàÿ    │âîäà äèñòèëëèðîâàííàÿ         │1000,0 ìë (êóá. ñì)│

    │(Êàëèíà Ã.Ï., Ãðàôîâà │ñóëüôàò ìàãíèÿ                │0,2 ã             

    │Ã.È., 1980)           │äâóçàìåùåííûé ôîñôîðíîêèñëûé  │1,0 ã             

                          │êàëèé                                            

                          │õëîðèä íàòðèÿ                 │5,0 ã             

│31. │Ñðåäà "À-2" ïëîòíàÿ   │âîäà äèñòèëëèðîâàííàÿ         │1000,0 ìë (êóá. ñì)│

    │(Êàëèíà Ã.Ï., Ãðàôîâà │àãàð-àãàð                     │20,0 ã            

    │Ã.È., 1980)           │ñóëüôàò ìàãíèÿ                │0,2 ã             

                          │îäíîçàìåùåííûé ôîñôîðíîêèñëûé │0,1 ã             

                          │ìàãíèé                                          

                          │êðàõìàë                       │5,0 ã             

                          │ðàñòâîðèìûé æåëàòèíà          │50,0 ã            

│32. │Êðîâÿíîé àãàð         │ìÿñîïåïòîííûé àãàð            │50,0 ã            

                          │ïåïòîí                        │10,0 ã            

                          │NaCl                          │5,0 ã             

                          │àãàð-àãàðà                    │30,0 ã            

                          │äåôèáðèíèðîâàííàÿ êðîâü       │50,0 ìë (êóá. ñì) 

│33. │Æåë÷íûé áóëüîí 20%    │ìÿñîïåïòîííûé áóëüîí          │800,0 ìë (êóá. ñì) │

                          │æåë÷è                         │200,0 ìë (êóá. ñì) │

│34. │Ñðåäà Ñàáóðî, ÃÎÑÒ    │ñóõîé ïåïòîí ôåðìåíòàòèâíûé   │200,0 ã           

    │10400-75 "Êîíñåðâû"   │ãëþêîçà èëè ìàëüòîçà          │800,0 ã           

                          │àãàð-àãàð                     │360,0 ã           

                          │âîäà äèñòèëëèðîâàííàÿ         │1000,0 ìë (êóá. ñì)│

│35. │Ñðåäà Êèòòè - Òàðîööè,│ïå÷åíü                        │300,0 ã           

    │ÃÎÑÒ 21237-75 "Ìÿñî"  │ðûáíûé áóëüîí                 │10 ë (êóá. äì)    

                          │ãëþêîçà                       │100,0 ã           

│36. │Ñðåäà Êëàðêà          │ïåïòîí                        │7,0 ã             

                          │ãëþêîçà                       │5,0 ã             

                          │K HPO                         │5,0 ã             

                          │ 2   4                                          

                          │äèñòèëëèðîâàííàÿ âîäà         │1,0 ë (êóá. äì)   

                          │àãàð-àãàð                     │2,0 ã             

│37. │Ôåíèëàëàíèíîâûé àãàð  │ôåíèëàëàíèí                   │2,0 ã             

                          │äðîææåâîé ýêñòðàêò            │0,1 ë (êóá. äì)   

                          │Na HPO  x 12H O               │1,0 ã             

                            2   4      2                                  

                          │NaCl                          │5,0 ã             

                          │àãàð (Äèôêî)                  │12,0 ã            

                          │äèñòèëëèðîâàííàÿ âîäà         │1,0 ë (êóá. äì)   

│38. │Ñðåäà ñ ìàëîíàòîì     │äðîææåâîé ýêñòðàêò            │10,0 ã            

    │íàòðèÿ                │(NH )O                        │20,0 ã            

                             4  4                                         

                          │KH PO                         │0,4 ã             

                            2  4                                          

                          │K HPO                         │0,5 ã              

                          │ 2   4                                          

                          │NaCl                          │2,0 ã             

                          │ìàëîíàò íàòðèÿ                │3,0 ã             

                          │äèñòèëëèðîâàííàÿ âîäà         │1,0 ë (êóá. äì)   

                          │áðîìòèìîëîâûé ñèíèé           │0,25 ã            

                          │ãëþêîçà                       │0,25 ã            

│39. │Ñðåäà ñ ëèçèíîì (äëÿ  │ïåïòîí                        │5,0 ã             

    │îïðåäåëåíèÿ           │äðîææåâîé ýêñòðàêò            │30,0 ã            

    │ëèçèíäåêàðáîêñèëàçû)  │ãëþêîçà                       │1,0 ã             

                          │áðîìêðåçîëïóðïóð (1,6% ñïèðò) │0,6 ã             

                          │êðåçîëðîò (0,1% ñïèðòà)       │5,0 ìë (êóá. ñì)  

                          │âîäà äèñòèëëèðîâàííàÿ         │1,0 ë (êóá. äì)   

                          │ëèçèí                         │0,5%              

│40. │Ãëèöåðèíîâàÿ ñìåñü    │ãëèöåðèí õ÷                   │1,6 ë (êóá. äì)   

    │(êîíñåðâèðóþùàÿ       │NaCl                          │18,9 ã            

    │æèäêîñòü Òèãå â       │2-çàìåùåííûé ôîñôîðíîêèñëûé   │250,0 ã           

    │ìîäèôèêàöèè Çàêñà) -  │íàòðèé (Na HPO )                                

    │5,0 ë (êóá. äì)                 2   4                                 

│41. │Ñðåäà ñ äóëüöèòîì     │ïåïòîí                        │5,0 ã             

                          │äðîææåâîé ýêñòðàêò            │30,0 ã            

                          │ãëþêîçà                       │1,0 ã             

                          │áðîìêðåçîë-ïóðïóð (1,6% ñïèðò)│0,6 ã              

                          │êðåçîëðîò (0,1% ñïèðò)        │5,0 ìë            

                          │âîäà äèñòèëë.                 │1,0 ë (êóá. äì)   

                          │äóëüöèò                       │0,5%              

│42. │Ñðåäà Ñåðîâà (æèäêàÿ) │ïåïòîí ôåðìåíòàò.             │10,0 ã            

                          │õëîðèñòûé íàòðèé              │8,5 ã             

                          │ãëþêîçà õ÷                    │97,5 ã            

                          │ôåíîëðîò 0,1% ð-ð             │10,0 ìë (êóá. ñì) 

                          │NaOH 20% ð-ð                  │10,0 ìë (êóá. ñì) 

                          │äèñòèëëèðîâàííàÿ âîäà         │1,0 ë (êóá. äì)   

│43. │Ñðåäà Ñåðîâà (ïëîòíàÿ)│ñóõîé ïèòàò. àãàð             │3,5 - 5,0 ã       

                          │ãëþêîçà                       │5,0 ã             

                          │ìî÷åâèíà                      │1,0 ã             

                          │ìîëèáäåíîâîêèñëûé àììîíèé     │1,0 ã             

                          │óãëåêèñëûé íàòðèé áåçâîäíûé   │1,0 ã             

                          │æåë÷ü êðóïíîãî ðîãàòîãî ñêîòà,│20,0 ìë (êóá. ñì) 

                          │30% âîäíûé ð-ð                                  

                          │êîíãîðîò 1,6% âîäíûé ð-ð      │9,0 ìë (êóá. ñì)  

                          │äèñòèëëèðîâàííàÿ âîäà         │1,0 ë (êóá. äì)   

│44. │Õëîðèñòî-ìàãíèåâàÿ    │ìàãíèé õëîðèñòûé              │39,0 ã            

    │ñðåäà                 êðèñòàëëè÷åñêèé                                 

                          │íàòðèé õëîðèñòûé              │8,0 ã             

                          │êàëèé ôîñôîðíîêèñëûé          │1,6 ã              

                          │îäíîçàìåùåííûé                                  

                          │ïåïòîí 10% ðàñòâîð            │50,0 ìë (êóá. ñì) 

                          │äðîææåâîé ýêñòðàêò            │22,0 ìë (êóá. ñì) 

                          │áðèëëèàíòîâûé çåëåíûé 0,1%    │5,0 ìë (êóá. ñì)  

                          │âîäíûé ð-ð                                      

│45. │Ñðåäà Êîçåðà          │NH H PO                       │1,5 ã             

                            4 2  4                                        

                          │K HPO                         │1,0 ã             

                          │ 2   4                                          

                          │MoO  x 7H O                   │0,2 ã             

                             4     2                                      

                          │Na-öèòðàò                     │2,77 ã            

                          │äèñòèëëèðîâ. âîäà             │1000,0 ìë (êóá. ñì)│

└────┴──────────────────────┴──────────────────────────────┴───────────────────┘

 

 

 

 

 

Ïðèëîæåíèå 3

 

ÐÅÀÊÒÈÂÛ, ÍÀÈÁÎËÅÅ ×ÀÑÒÎ ÓÏÎÒÐÅÁËßÅÌÛÅ

 ÁÀÊÒÅÐÈÎËÎÃÈ×ÅÑÊÈÕ ËÀÁÎÐÀÒÎÐÈßÕ

 

┌────┬──────────────────────────────────────────┬────────────┬─────────────────┐

│ N                 Íàèìåíîâàíèå               │Êâàëèôèêàöèÿ│   ÃÎÑÒ èëè ÒÓ  

ï/ï │                                                                      

├────┼──────────────────────────────────────────┼────────────┼─────────────────┤

│ 1                      2                           3             4       

├────┼──────────────────────────────────────────┼────────────┼─────────────────┤

│1.  Àììîíèé-æåëåçî (II) ñåðíîêèñëûé (ñîëü     │÷äà, õ÷     │ÃÎÑÒ 4208-72    

    │Ìîðà)                                                                 

│2.  │Àììîíèé ôîñôîðíîêèñëûé îäíîçàìåùåííûé     ÷, ÷äà, õ÷  │ÃÎÑÒ 3771-74    

│3.  │Àììîíèé ôîñôîðíîêèñëûé äâóçàìåùåííûé      │÷, ÷äà, õ÷  │ÃÎÑÒ 3772-74    

│4.  │Àóðèí, èíäèêàòîð ("ðîçîëîâàÿ" êèñëîòà)    │÷äà         │ÒÓ 6-09-1091-76 

│5.  │Áðèëëèàíòîâûé çåëåíûé                     ÷           │ÒÓ 6-09-4278-76 

│6.  │Áðîìòèìîëîâûé ñèíèé, èíäèêàòîð            │÷äà         │ÒÓ 6-09-2086-77 

│7.  │Áðîìòèìîëîâûé ñèíèé, âîäîðàñòâîðèìûé,     │÷äà         │ÒÓ 6-09-3045-77 

    │èíäèêàòîð                                                             

│8.  │Ãëèöåðèí                                  │÷äà         │ÃÎÑÒ 6259-75    

│9.  ï-(Äèìåòèëàìèíî)-áåíçàëüäåãèä             │÷äà, ÷      │ÒÓ 6-09-3272-77 

│10. │Æåëåçî (III) ëèìîííîêèñëîå, âîäíîå        ÷           │ÒÓ 6-09-2562-77 

│11. │Æåëåçî õëîðíîå                            │÷äà, ÷, õ÷  │ÃÎÑÒ 4147-74    

│12. │Éîä                                       │÷äà         │ÃÎÑÒ 4159-79    

│13. │Êàëèé åäêèé (êàëèÿ ãèäðàò îêèñè)                      │èìï.            

│14. │Êàëèé éîäèñòûé                            │÷äà         │ÃÎÑÒ 4232-74    

│15. │Êàëèé ñåðíîêèñëûé (ñóëüôàò êàëèÿ)         │÷äà         │ÃÎÑÒ 4145-74    

│16. │Êàëèé òåëëóðèñòîêèñëûé, âîäíûé            ÷           │ÒÓ 6-09-2060-77 

│17. │Êàëèé ôîñôîðíîêèñëûé îäíîçàìåùåííûé       │õ÷          │ÃÎÑÒ 4198-75    

│18. │Êàëèé ôîñôîðíîêèñëûé äâóçàìåùåííûé,       │÷äà         │ÃÎÑÒ 2493-75    

    │3-âîäíûé                                                              

│19. │Êàëüöèé õëîðèñòûé                         ÷           │ÃÎÑÒ 4460-77    

│20. │Êèñëîòà ñîëÿíàÿ êîíöåíòðèðîâàííàÿ         │-           │ÃÎÑÒ 3118-77     

│21. │Êèñëîòà ôîñôîðíàÿ êîíöåíòðèðîâàííàÿ,      │-           │ÃÎÑÒ 6552-80    

    │î÷èùåííàÿ                                                             

│22. │Êðåçîëîâûé êðàñíûé, èíäèêàòîð             │÷äà         │ÃÎÑÒ 5849-74    

│23. │Êðåçîëîâûé êðàñíûé, âîäîðàñòâîðèìûé,      │÷äà         │ÒÓ 6-09-796-76  

    │èíäèêàòîð                                                             

│24. │Ìàãíèé ìîëèáäåíîâîêèñëûé, 5-âîäíûé        │÷           │ÒÓ 6-09-01-244-74│

│25. │Ìàãíèé ñåðíîêèñëûé, 7-âîäíûé              │õ÷          │ÃÎÑÒ 4523-77    

│26. │Ìàãíèé õëîðèñòûé, 6-âîäíûé                │÷äà         │ÃÎÑÒ 4209-77    

│27. │Ìåäü (II) ñåðíîêèñëàÿ, 5-âîäíàÿ           │÷äà         │ÃÎÑÒ 4165-78    

│28. │Ìåòèëâèîëåò                               ÷           │ÒÓ 6-09-15-424-79│

│29. │Ìåòèëåíîâûé ñèíèé                         │÷äà         │ÒÓ 6-09-29-76   

│30. │Ìåòèëîâûé êðàñíûé, èíäèêàòîð (ìåòèëðîò)   │÷äà         │ÃÎÑÒ 5853-51    

│31. │Ìî÷åâèíà                                  │÷äà         │ÃÎÑÒ 6691-77    

│32. │Íàòðèé-àììîíèé ôîñôîðíîêèñëûé,            │÷äà         │ÃÎÑÒ 4170-78    

    │äâóçàìåùåííûé, 4-âîäíûé                                               

│33. │Íàòðèÿ ãèäðîîêèñü                         ÷           │ÃÎÑÒ 4328-77    

│34. │Íàòðèé ëèìîííîêèñëûé òðåõçàìåùåííûé       │÷äà         │ÃÎÑÒ 22280-76   

│35. │Íàòðèé ìàëîíîâîêèñëûé (íàòðèÿ ìàëîíàò)    │÷           │ÒÓ 6-09-4921-80 

│36. │Íàòðèé ñåëåíèñòîêèñëûé (íàòðèÿ ñåëåíèò)   ÷           │ÒÓ 6-09-1315-76 

│37. │Íàòðèé óãëåêèñëûé áåçâîäíûé               ÷, ÷äà, õ÷  │ÃÎÑÒ 83-79      

│38. │Íàòðèé ñåðíîâàòèñòîêèñëûé (íàòðèé         │÷, ÷äà      ÑÒ ÑÝ 223-75   

    │òèîñóëüôàò)                                                           

│39. │Íàòðèé ôîñôîðíîêèñëûé îäíîçàìåùåííûé,     ÷, ÷äà      │ÃÎÑÒ 245-76     

    │2-âîäíûé                                                              

│40. │Íàòðèé ôîñôîðíîêèñëûé äâóçàìåùåííûé       │÷, ÷äà      │ÃÎÑÒ 11773-76   

│41. │Íàòðèé õëîðèñòûé                          │÷äà         │ÃÎÑÒ 4233-77    

│42. │1-Íàôòîë (àëüôà-Íàôòîë)                   ÷, ÷äà      │ÃÎÑÒ 5838-79    

│43. │ì-Íèòðîôåíîë, èíäèêàòîð                   │÷äà         │ÒÓ 6-09-07-294-74│

│44. │ï-Íèòðîôåíîë                              │÷, ÷äà      │ÒÓ 6-09-3973-74 

│45. │Ñâèíåö óêñóñíîêèñëûé                      │÷äà         │ÃÎÑÒ 1027-67    

│46. │Ñïèðò àìèëîâûé                            ÷           │ÒÓ 6-09-3467-79 

│47. │Ñïèðò ýòèëîâûé 96°                        │-           │ÃÎÑÒ 5962-67    

│48. │Ôåíîë (êàðáîëîâàÿ êèñëîòà êðèñòàëëè÷åñêàÿ)│-           │ÃÎÑÒ 6417-72    

│49. │Ôåíîëîâûé êðàñíûé                         │-           │ÃÎÑÒ 4599-73    

│50. │Ôóêñèí êèñëûé                             ÷           │ÒÓ 6-09-3803-74 

│51. │Ôóêñèí îñíîâíîé äëÿ ìèêðîáèîëîãè÷åñêèõ    │÷           │ÒÓ 6-09-3804-75 

    │öåëåé                                                                 

│52. │Ôóêñèí îñíîâíîé äëÿ ñóõèõ ïèòàòåëüíûõ ñðåä│÷äà         │ÒÓ 6-09-3236-78 

└────┴──────────────────────────────────────────┴────────────┴─────────────────┘

 

 







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